The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.25µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
RelevanceDendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.
Flow cytometry staining of BALB/c splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC and either 0.125 µg of ab95767 (right) or 0.125 µg of Rat IgG2b ? Isotype Control PE (left). Total viable cells were used for analysis.
References for Anti-Dendritic cells antibody [33D1] (Phycoerythrin) (ab95767)