The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 2µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceDendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.
ab112196 at 2µg/106 cells staining Dendritic cells in Rat Thymus cells (Wistar strain) by Flow Cytometry.
Percentage of cells stained above control (FITC Mouse IgG1): 82.8%.
References for Anti-Dendritic cells antibody [MRC OX-62] (FITC) (ab112196)
This product has been referenced in:
Chen-Woan M et al. A new protocol for the propagation of dendritic cells from rat bone marrow using recombinant GM-CSF, and their quantification using the mAb OX-62. J Immunol Methods178:157-71 (1995).
Read more (PubMed: 7836778) »
Brenan M & Puklavec M The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin. J Exp Med175:1457-65 (1992).
Read more (PubMed: 1588275) »