Anti-Dendritic cells antibody [MRC OX-62] (FITC) (ab112196)

Overview

  • Product nameAnti-Dendritic cells antibody [MRC OX-62] (FITC)
    See all Dendritic cells primary antibodies
  • Description
    Mouse monoclonal [MRC OX-62] to Dendritic cells (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat PVG density gradient-enriched veiled cells.

  • Positive control
    • Wistar Rat dendritic cells from thymus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab112196 is purified from ascitic fluid via Protein G Chromatography.
  • ClonalityMonoclonal
  • Clone numberMRC OX-62
  • MyelomaNS0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceDendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response.
  • Alternative names
    • DC specific surface marker antibody
    • DCs antibody
    • Dendritic cell antibody
    • Dendritic Cell Marker antibody

Anti-Dendritic cells antibody [MRC OX-62] (FITC) images

  • ab112196 at 2µg/106 cells staining Dendritic cells in Rat Thymus cells (Wistar strain) by Flow Cytometry.
    Percentage of cells stained above control (FITC Mouse IgG1): 82.8%.

References for Anti-Dendritic cells antibody [MRC OX-62] (FITC) (ab112196)

This product has been referenced in:
  • Chen-Woan M  et al. A new protocol for the propagation of dendritic cells from rat bone marrow using recombinant GM-CSF, and their quantification using the mAb OX-62. J Immunol Methods 178:157-71 (1995). Read more (PubMed: 7836778) »
  • Brenan M & Puklavec M The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin. J Exp Med 175:1457-65 (1992). Read more (PubMed: 1588275) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"