Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349)

Overview

  • Product nameAnti-Dengue Virus E glycoprotein antibody [DE1]
    See all Dengue Virus E glycoprotein primary antibodies
  • Description
    Mouse monoclonal [DE1] to Dengue Virus E glycoprotein
  • SpecificityThis antibody reacts with a conformational epitope on the envelope gylcoprotein of Dengue Virus 1, 2, 3 and 4.
  • Tested applicationsSuitable for: ICC/IF, ELISA, Electron Microscopy, Dot blot, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Dengue Virus 1, Dengue Virus 2, Dengue Virus 3, Dengue Virus 4
  • Immunogen

    Full length native protein purified from Dengue Virus 2 (16681) infected supernatant

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 10mM Sodium Azide
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberDE1
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5 - 1/20.
ELISA Use at an assay dependent concentration. This antibody has been used in ELISA for the detection of degue IgM in patient serum samples.
Electron Microscopy Use at an assay dependent concentration. PubMed: 24681578
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 24625456ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceDengue virus E glycoprotein is thought to be essential for membrane fusion and is also involved in binding to cellular receptors. It is the primary antigen responsible for inducing the protective immune response.
    • Database links
      • Alternative names
        • Dengue E glycoprotein antibody
        • Dengue envelope glycoprotein antibody
        • Dengue glycoprotein E antibody
        • Dengue virus envelope glycoprotein antibody
        • Dengue virus glycoprotein E antibody
        see all

      Anti-Dengue Virus E glycoprotein antibody [DE1] images

      • Dengue Virus 2 infected Vero cells stained with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.
      • Dengue Virus 2 infected Vero cells stained according to protocol with ab41349 antibody against Dengue Virus envelope glycoprotein E (green). Cell nuclei are blue.

      References for Anti-Dengue Virus E glycoprotein antibody [DE1] (ab41349)

      This product has been referenced in:
      • Al-Alimi AA  et al. Dengue virus type 2 (DENV2)-induced oxidative responses in monocytes from glucose-6-phosphate dehydrogenase (G6PD)-deficient and G6PD normal subjects. PLoS Negl Trop Dis 8:e2711 (2014). Flow Cyt . Read more (PubMed: 24625456) »
      • Burlaud-Gaillard J  et al. Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles. PLoS One 9:e93573 (2014). Electron Microscopy . Read more (PubMed: 24681578) »

      See all 4 Publications for this product

      Product Wall

      Thank you for your enquiry. Basically, the epitopes for DE1 and DE2 are complex, conformational epitopes and probably non-linear - and since the immunogen is not a peptide, the amino acid sequence is not known. The information on the recombinan...

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      Unfortunately, we do not know where the epitope for DE1 is on the E glycoprotein. However, we do know that it does not bind recombinant DIII from the pET expression system. This therefore suggests that this antibody will not recognise endogenous domain...

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      Thank you for contacting us.

      We searched on our web page and there are three products that I could recommend to you. They are all three against the IgG2b of mouse and were used for ICC/IF. The species the three products were raised in is diff...

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      Thank you for contacting us.

      ab41349 is a tissue culture supernatant therefore it is normal for it to be reddish as this may be due to the colour of the media. It is likely that there are additional reagents and/or proteins that might oblitera...

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      Thank you for your response.

      Currently, we have antibodies against Dengue virus which detect Type 1 and Type 3 separately.

      If you need any further assistance, please do not hesitate to contact me.

      Thank you for your enquiry and your interest in our products.

      I can confirm that the Western blot results with this antibody (ab41349: Anti-Dengue Virus E glycoprotein antibody [DE1]) have been inconclusive, thus we do not list WB as a tested...

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      Thank you for your enquiry. DE1 (ab41349) and DE2 (ab41696) are monoclonal antibodies from two different fusion experiments but the immunizing protocol is the same for both fusions. As mentioned in the datasheets, although they are both dengue grou...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"