Anti-Dengue Virus NS1 glycoprotein antibody [DN1] (ab41490)

Overview

  • Product nameAnti-Dengue Virus NS1 glycoprotein antibody [DN1]
    See all Dengue Virus NS1 glycoprotein primary antibodies
  • Description
    Mouse monoclonal [DN1] to Dengue Virus NS1 glycoprotein
  • SpecificityRecognises the non-structural protein NS1 from Dengue Virus 2 (16681 strain). Reacts only weakly with NS1 from Dengue Virus 2 NGC strain, use ab41623 for detection of Dengue Virus 2 NGC NS1.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Dengue Virus 2
  • Immunogen

    Full length native protein purified from Dengue Virus 2 infected supernatant

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 10mM Sodium Azide
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberDN1
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Use under non reducing condition. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP Use at an assay dependent dilution.
ICC/IF 1/5 - 1/20.

Target

  • RelevanceNS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
  • Database links
    • Alternative names
      • Dengue NS1 antibody
      • Dengue Virus non structural protein 1 antibody

    Anti-Dengue Virus NS1 glycoprotein antibody [DN1] images

    • All lanes : Anti-Dengue Virus NS1 glycoprotein antibody [DN1] (ab41490)

      Lane 1 : Unheated lysate from C6/36 cells infected with Dengue Virus 2 (NGC serotype)
      Lane 2 : Boiled lysate from C6/36 cells infected with Dengue Virus 2 (NGC serotype)
      Lane 3 : Unheated lysate from C6/36 cells infected with Dengue Virus 2 (16681 serotype)
      Lane 4 : Boiled lysate from C6/36 cells infected with Dengue Virus 2 (16681 serotype)


      Predicted band size : 40 kDa
      Observed band size : 40,80 kDa (why is the actual band size different from the predicted?)

    References for Anti-Dengue Virus NS1 glycoprotein antibody [DN1] (ab41490)

    ab41490 has not yet been referenced specifically in any publications.

    Product Wall

    Thank youand your customer very much for your interest in anti dengue virusNS1 antibodies.

    I can confirm that we have three in our catalog: ab41490, ab41616 and ab41623.

    To our knowledge, none of these antibodies has been tested in...

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    Thank youfor your inquiry.

    I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

    Isotype control are not included and have to be purchased separately.

    ab41616 (Anti-Dengue Virus NS1 glyc...

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    Thank you for your reply. These three antibodies are provided as tissue culture supernatant. This is stated in the purity section of the datasheet. It has some sodium azide added to it, but is not provided in a storage buffer. I am sorry we do not know...

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    Thank you for your enquiry. Wecan only confirm that ab41490 DN1 antibody will recognize NS1 protein from an MVA NS1 recombinant.I am sorry, we have no data from NS1 expressed in E coli or P pastoris However, if your recombinant proteins (ie NS1 from DE...

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    Thank you for contacting Abcam. The culture supernatants are from hybridoma cultures and since it is not purified we do not have an estimate of the concentration of the mab in the supernatant which also contains other proteins. I am sorry that I was no...

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    For ab41490, you get a total of 1ml of solution per order. If there is anything else I can help you with, please let me know.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"