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Dengue Virus NS1 glycoprotein protein (His tag) (ab64456)

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Overview

  • Product nameDengue Virus NS1 glycoprotein protein (His tag)
  • Protein descriptionRecombinant full length protein (Dengue Virus)
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab64456 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA
    WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.
    Lateral flow (immunochromatographic strip -ICS tests).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Dengue NS1Dengue Virus non structural protein 1
  • RelevanceNS1 is one of 7 Dengue Virus non-structural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.

    • Dengue Virus NS1 glycoprotein protein (His tag) images

    • SDS-PAGE - Dengue Virus NS1 glycoprotein protein (His tag) (ab64456)
      SDS-PAGE - Dengue Virus NS1 glycoprotein protein (His tag) (ab64456)

    References for Dengue Virus NS1 glycoprotein protein (His tag) (ab64456)

    ab64456 has not yet been referenced specifically in any publications.

    Publishing research using ab64456 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab64456 Submit a Question for ab64456

    Displaying 1 - 10 of 20 results for Abreviews and Q&A

    10
    Votes: Highest First

    I'd like to ask information about the dengue ns1. I am wondering how recombinant ns1 is purified e.g. HPLC. Is it a dimer, monomer or polymer? What is its molecular weight? If I have to do the western blot, do I need to do heat/reducing condition?
    <...

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    This protein is purified by affinity chromatography.

    On a gel, it can be seen as a monomer, dimer and polymer. We usually recommend heating samples up to 95ºC in SDS-Page before loading and running the gel in reducing conditions.

    ...

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    Please advise if there is way that end user can run this product to raise as immunogen to raise an antibody. How abcam will help this researcher, in this case what are the possibilities?



    Sincere regards,

    Thank you for your new contact.

    As I have explained in my previous e-mail, ab64456 has never been used as immunogen.

    Since this glycoprotein is epitope tagged (having additional amino acids ie.HA sequence: YPYDVPDYA) and the storage bu...

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    I would like to detect Dengue NS1 virus using microfluidics platform. I am looking for Dengue NS1 capture antibodies, antigens and detection antibodies. I looked in to some of the items listed from your website. They are:
    ab41490, ab41616, ab41623, a...

    Read More

    Thank you for your interest in our products.

    The two kits we have for detection of Dengue virus-specific IgG or IgM ab108728 and ab108729, are for detection of antibodies in serological samples that detect Dengue virus. So, they detect an immune...

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    Dear All,

    Please advise if the product ab64456 can used to raised to use as the Immunogen. Please advise by return at the earliest.

    Thanks and best regards,

    Thank you for your enquiry regarding ab64456.

    It is a Dengue Virus NS1 glycoprotein protein (His tag) which has been tested and characterised for ELISA and WB applications. It has not been used as immunogen to raise an antibody against.
    Read More

    What is the strain name of this Dengue Virus e.g. Strain Thailand, strain Jamaica etc.

    Thank you for contacting us. I apologize for the delay.

    The ab64456 Dengue NS1is from serotype 2, Thailand strain.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information...

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    What is the strain name of this Dengue Virus e.g. Strain Thailand, strain Jamaica etc.

    My customer would like to know the strain name of Dengue virus protein is from. Could you provide this info?

    Many thanks in advance!

    Dear Sir/ Madam,
    We are trying to develop and ELISA for Dengue NS1 detection and having been working with your products in some of our previous and other present work, we have considered using your dengue products as well. However, during our prelimi...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable th...

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    Buenos días ,
    te hago llegar los comentarios de mis compañeros.
    We will very much appreciate if you can provide us details on the epitope targeted byab41616 and also if it has been compromised in the his tag region ofab64456 so we ...

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    Gracias por tu respuesta.
    He consultado esta información con mis compañeros del laboratorio.
    La glicoproteína (ab64456) contiene toda la secuencia excepto el dominio transmembranal. Además contiene 2x6 His tags, uno localizado en el extremo N te...

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    Dear Sir/Madam,
    We are trying to develop an ELISA for Dengue NS1 detection and since we have been been working for a long time with your products, we have decided to use your dengue products. We are in the process of validating our immunocapture syst...

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    Thank you for contacting us.
    I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, but ...

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    Good afternoon,
    Thank you for the information on the molecular weight.
    Does the polyclonal antibody you mentioned ab9200 reacts with the NS1 antigen?
    because what we are needing is a rabbit polyclonal anti-dengue NS1 which is cross reactive...

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    I am sorry for this misunderstanding.

    We do not know which protein ab9200 exactly recognises and therefore I suspect this antibody will not be suitable for your purposes.

    We onlyhave a mouse monoclonalantibody against Dengue Virus NS1 ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"