You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-DENN antibody (ab2043)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-DENN antibody
See all DENN products (2) ...

Description

Rabbit polyclonal to DENN

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Peptide corresponding to amino acids 1570 to 1588 of human DENN (1). The sequence of immunogenic peptideis identical to that of DENN and differs by one amino acidwith rat GDP/GTP exchange protein Rab3-GEP (2,5).

Epitope

AAVHSSEEDLRTPPRPVSS

Positive control

HeLa whole cell lysate or 3T3 whole cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> TRADD
Cancer >> Invasion/microenvironment >> Apoptosis >> Death receptors & ligands >> FADD
Signal Transduction >> Metabolism >> Mitochondrial

  • Western blot - Anti-DENN antibody (ab2043)Western blot - Anti-DENN antibody (ab2043) image (enlarge)

  • Immunocytochemistry - Anti-DENN antibody (ab2043)Immunocytochemistry - Anti-DENN antibody (ab2043) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.

Tissue specificity

Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.

Sequence similarities

Belongs to the MADD family.
Contains 1 dDENN domain.
Contains 1 death domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ8WXG6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLJ35600 antibody
  • Differentially expressed in normal and neoplastic cells antibody
  • IG20 antibody
  • Insulinoma glucagonoma clone 20 antibody
  • KIAA0358 antibody
  • MADD antibody
  • MADD_HUMAN antibody
  • MAP kinase-activating death domain protein antibody
  • Rab3 GDP/GTP exchange factor antibody
  • RAB3GEP antibody
see all

Database links

Anti-DENN antibody images:

  Western blot - Anti-DENN antibody (ab2043)

Western blot - Anti-DENN antibody (ab2043)

All lanes : Anti-DENN antibody (ab2043) at 1/250 dilution

Lane 1 : Hela whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NIH3T3 whole cell lysate


Predicted band size : 220 kDa

  Immunocytochemistry - Anti-DENN antibody (ab2043)

Immunocytochemistry - Anti-DENN antibody (ab2043)

ab2043 at 10µg/ml staining DENN in human spleen tissue by ICC

References for Anti-DENN antibody (ab2043)

ab2043 has not yet been referenced specifically in any publications.

Publishing research using ab2043? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"