Overview

  • Product name
  • Description
    Rabbit polyclonal to DENN
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human DENN aa 1570-1588.
    Sequence:

    AAVHSSEEDLRTPPRPVSS


    (Peptide available as ab8384)

  • Positive control
    • HeLa whole cell lysate or 3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/250 - 1/500. Predicted molecular weight: 220 kDa.Can be blocked with DENN peptide (ab8384).

Target

  • Function
    Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
  • Tissue specificity
    Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines.
  • Sequence similarities
    Belongs to the MADD family.
    Contains 1 dDENN domain.
    Contains 1 death domain.
    Contains 1 DENN domain.
    Contains 1 uDENN domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Differentially expressed in normal and neoplastic cells antibody
    • FLJ35600 antibody
    • IG20 antibody
    • Insulinoma glucagonoma clone 20 antibody
    • KIAA0358 antibody
    • MADD antibody
    • MADD_HUMAN antibody
    • MAP kinase-activating death domain protein antibody
    • Rab3 GDP/GTP exchange factor antibody
    • RAB3GEP antibody
    see all

Images

  • All lanes : Anti-DENN antibody (ab2043) at 1/250 dilution

    Lane 1 : Hela whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : NIH3T3 whole cell lysate


    Predicted band size : 220 kDa
  • ab2043 at 10µg/ml staining DENN in human spleen tissue by ICC
  • Immunofluorescence of MADD in Hela cells using ab2043 at 20 ug/ml.

References

ab2043 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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