1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.
Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.