Overview

  • Product name
  • Description
    Rabbit polyclonal to DENND1B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Zebrafish
  • Immunogen

    Synthetic peptide: PVNLSVNQEI FIACEQVLKD QPALVPHSYF IAPDVTGLPT IPESRNLTEY , corresponding to a region within internal sequence amino acids 144 - 193 of Human DENND1B isoform 1 (NP_659414)

  • Positive control
    • 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.
  • Tissue specificity
    Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.
  • Sequence similarities
    Contains 1 dDENN domain.
    Contains 1 DENN domain.
    Contains 1 uDENN domain.
  • Post-translational
    modifications
    Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.
  • Cellular localization
    Cytoplasm > cytosol. Cytoplasmic vesicle > clathrin-coated vesicle.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1ORF18 antibody
    • C1orf218 antibody
    • Chromosome 1 open reading frame 218 antibody
    • Connecdenn 2 antibody
    • DEN1B_HUMAN antibody
    • DENN domain containing protein 1B antibody
    • DENN domain-containing protein 1B antibody
    • DENN MADD domain containing 1B antibody
    • DENND1B antibody
    • DKFZp547O0715 antibody
    • FAM31B antibody
    • Family with sequence similarity 31 member B antibody
    • FLJ20054 antibody
    • FLJ21179 antibody
    • FLJ33888 antibody
    • MGC103810 antibody
    • MGC27044 antibody
    • Protein FAM31B antibody
    • RP11 53I24.2 antibody
    see all

Images

  • Anti-DENND1B antibody (ab84347) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References

ab84347 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84347.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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