Overview

  • Product nameAnti-DENND5A antibody
    See all DENND5A primary antibodies
  • Description
    Rabbit polyclonal to DENND5A
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between internal sequence amino acids 702-732 of Human DENND5A (NP_056028.2).

  • Positive control
    • HeLa cell lysate. IHC-P: FFPE human cerebral cortex tissue sections. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 147 kDa.

Target

  • FunctionMay be involved in Rab6-mediated GTPase signaling.
  • Sequence similaritiesBelongs to the RAB6IP1 family.
    Contains 1 dDENN domain.
    Contains 1 DENN domain.
    Contains 1 PLAT domain.
    Contains 2 RUN domains.
    Contains 1 uDENN domain.
  • Information by UniProt
  • Database links
  • FormCellular localisation Golgi apparatus, Membrane from mouse swissprot.
  • Alternative names
    • DEN5A_HUMAN antibody
    • DENN domain-containing protein 5A antibody
    • DENN/MADD domain containing 5A antibody
    • Dennd5a antibody
    • FLJ22354 antibody
    • FLJ33829 antibody
    • FLJ43455; antibody
    • KIAA1091 antibody
    • RAB6 interacting protein 1 antibody
    • Rab6-interacting protein 1 antibody
    • Rab6IP1 antibody
    see all

Anti-DENND5A antibody images

  • ICC/IF image of ab107678 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107678 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of DENND5A staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107678, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-DENND5A antibody (ab107678) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 147 kDa

References for Anti-DENND5A antibody (ab107678)

ab107678 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 7.5%)
Sample Dog Cell lysate - whole cell (kidney)
Specification kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"