Overview

  • Product nameAnti-Deoxyguanosine kinase antibody
    See all Deoxyguanosine kinase primary antibodies
  • Description
    Rabbit polyclonal to Deoxyguanosine kinase
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH selected from the C-terminal region of human DGUOK.

  • Positive control
    • Mouse thymus tissue lysate, K562 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, eluted with both high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab38013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa.

Target

  • RelevanceMitochondrial deoxyguanosine kinase (DGUOK) is required for the phosphorylation of several deoxyribonucleosides and certain purine deoxykribonucleoside analogs widely employed as antiviral and chemotherapeutic agents. Purine deoxyribonucleoside analogs are extensively used in treatment of lymphoproliferative disorders. These compounds are administered as pro-drugs, and their efficiency is dependent on intracellular phosphorylation to the corresponding triphosphates. In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by 2 deoxyribonucleoside kinases: cytosolic deoxycytidine kinase (DCK) and mitochondrial deoxyguanosine kinase (DGUOK also known as DGK). DGUOK expression is ubiquitous, with highest levels in muscle, brain, liver and lymphoid tissues. Defects in DGUOK are a cause of mitochondrial DNA depletion syndrome (MDS). MDS is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. Primary mtDNA depletion is inherited as an autosomal recessive trait and may affect single organs, typically muscle or liver, or multiple tissues. Mitochondrial DNA depletion syndromes are phenotypically heterogeneous, autosomal recessive disorders characterized by tissue-specific reduction in mtDNA copy number. Affected individuals with the hepatocerebral form of mtDNA depletion syndrome have early progressive liver failure and neurologic abnormalities, hypoglycemia, and increased lactate in body fluids.
  • Database links
  • Alternative names
    • Deoxyguanosine kinase mitochondrial antibody
    • dGK antibody
    • DGUOK antibody

Anti-Deoxyguanosine kinase antibody images

  • All lanes : Anti-Deoxyguanosine kinase antibody (ab38013) at 1/100 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : K562 cell lysate


    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References for Anti-Deoxyguanosine kinase antibody (ab38013)

This product has been referenced in:
  • Yamauchi T  et al. Cytarabine-resistant leukemia cells are moderately sensitive to clofarabine in vitro. Anticancer Res 34:1657-62 (2014). Human . Read more (PubMed: 24692694) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"