Recombinant fragment: SFCRQISPEE FEYQRSYGSQ EPLAALLEEV ITDAKLSNKE KKKKLKQFQK SYPEVYQERF PTPESAALLF PEKPKPKPQL LMWALKKPFQ PFQRTRSFRM , corresponding to amino acids 430-530 of Human DEPDC1B with tag (NP_060839)
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 62 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.