Anti-DERL1/Derlin-1 antibody (ab176732)
Key features and details
- Rabbit polyclonal to DERL1/Derlin-1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DERL1/Derlin-1 antibody
See all DERL1/Derlin-1 primary antibodies -
Description
Rabbit polyclonal to DERL1/Derlin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Non human primates -
Immunogen
Synthetic peptide within Human DERL1/Derlin-1 aa 201-251. The exact sequence is proprietary. NP_077271.1.
Database link: Q9BUN8 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 cell lysates. IP: HeLa cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab176732 is affinity purified using an epitope specific to DERL1/Derlin-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 29 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 29 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Derlin 1 is a channel-like protein found in the endoplasmic reticulum (ER) membrane. It is specifically required for the degradation of misfolded ER luminal proteins. Derlin 1 participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. It may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol and then transferring them to the ATPase VCP complex, which translocates and ubiquitinates misfolded proteins. In the case of infection by cytomegaloviruses, derlin 1 plays a central role in export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Derlin 1 also participates in the degradation of misfolded cytomegalovirus US2 protein. -
Cellular localization
Endoplasmic reticulum; multi pass membrane protein. -
Database links
- Entrez Gene: 420350 Chicken
- Entrez Gene: 404121 Cow
- Entrez Gene: 475086 Dog
- Entrez Gene: 100727484 Guinea pig
- Entrez Gene: 79139 Human
- Entrez Gene: 67819 Mouse
- Entrez Gene: 100626802 Pig
- Entrez Gene: 362912 Rat
see all -
Alternative names
- DER 1 antibody
- DER1 antibody
- Der1 like protein 1 antibody
see all
Images
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All lanes : Anti-DERL1/Derlin-1 antibody (ab176732) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds -
ab176732 at 0.4 µg/ml detecting DERL1/Derlin-1 in HeLa whole cell lysate by WB following IP. ab176732 was used for IP at 3 µg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling DERL1/Derlin-1 using ab176732 at a 1/200 dilution, followed by peroxidase substrate detection.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab176732 has been referenced in 5 publications.
- Li S et al. Abnormally decreased renal Klotho is linked to endoplasmic reticulum-associated degradation in mice. Int J Med Sci 19:321-330 (2022). PubMed: 35165517
- McDonald EF et al. Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants. Mol Biol Cell 33:ar62 (2022). PubMed: 35389766
- Rao B et al. The cryo-EM structure of an ERAD protein channel formed by tetrameric human Derlin-1. Sci Adv 7:N/A (2021). PubMed: 33658201
- Zeng J et al. Derlin-1 exhibits oncogenic activities and indicates an unfavorable prognosis in breast cancer. Cell Biol Int 44:593-602 (2020). PubMed: 31670413
- Yang F et al. Potential contribution of ryanodine receptor 2 upregulation to cGMP/PKG signaling-induced cone degeneration in cyclic nucleotide-gated channel deficiency. FASEB J 34:6335-6350 (2020). PubMed: 32173907