The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 12 kDa.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.
Specifically and constitutively expressed in eccrine sweat gland cells. Secreted into the sweat at a concentration of 1-10 micrograms/ml.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human skin tissue labeling Dermcidin with ab175519 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells labeling Dermcidin with ab175519 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.