Anti-Dermcidin antibody - C-terminal (ab175519)

Overview

  • Product name
    Anti-Dermcidin antibody - C-terminal
    See all Dermcidin primary antibodies
  • Description
    Rabbit polyclonal to Dermcidin - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Dermcidin aa 81-110 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P81605

  • Positive control
    • T47D cell line lysate, Human skin tissue, 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 12 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat.
    Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.
  • Tissue specificity
    Specifically and constitutively expressed in eccrine sweat gland cells. Secreted into the sweat at a concentration of 1-10 micrograms/ml.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIDD antibody
    • DCD 1 antibody
    • dcd antibody
    • DCD-1 antibody
    • DCD_HUMAN antibody
    • DSEP antibody
    • HCAP antibody
    • PIF antibody
    • Preproteolysin antibody
    see all

Images

  • Anti-Dermcidin antibody - C-terminal (ab175519) at 1/1000 dilution + T47D cell line lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human skin tissue labeling Dermcidin with ab175519 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining. 

  • Flow cytometric analysis of 293 cells labeling Dermcidin with ab175519 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175519 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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