Overview

  • Product nameAnti-Desmin antibody
    See all Desmin primary antibodies
  • Description
    Rabbit polyclonal to Desmin
  • SpecificityIn immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.
  • Tested applicationsSuitable for: Electron Microscopy, IP, ELISA, WB, IHC-Frmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Syrian Hamster
  • Immunogen

    Full length protein (Human).

  • Positive control
    • Heart muscle, smooth and skeletal muscle, leiomyoma and rhabdomyomas
  • General notesProlonged fixation in buffered formalin can destroy the epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab8592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent concentration. PubMed: 17535849
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 53 kDa.
IHC-Fr Use at an assay dependent concentration.
  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in diseaseDefects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Anti-Desmin antibody images

    • ab8592 was used to stain mouse prostate.

    References for Anti-Desmin antibody (ab8592)

    This product has been referenced in:
    • Gerling M  et al. Stromal Hedgehog signalling is downregulated in colon cancer and its restoration restrains tumour growth. Nat Commun 7:12321 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27492255) »
    • Kostallari E  et al. Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence. Development 142:1242-53 (2015). IHC-Fr, ICC/IF . Read more (PubMed: 25742797) »

    See all 36 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 20 µg
    Specification Heart
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Aug 21 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue (Embryonic Mouse Heart Section)
    Permeabilization Yes - Tween-20
    Specification Embryonic Mouse Heart Section
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    Submitted Jul 25 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Mouse Cell (Pericytes (xenograft tumour tissue))
    Specification Pericytes (xenograft tumour tissue)
    Permeabilization No
    Fixative Formaldehyde
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    Submitted May 10 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Colon)
    Specification Colon
    Fixative Paraformaldehyde
    Permeabilization Yes - triton 0,1% in PBS
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 24°C
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    Dr. Alessandro Gandelli

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    Submitted Mar 01 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Intestine)
    Specification Intestine
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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    Submitted Aug 28 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Colon)
    Specification Colon
    Fixative Paraformaldehyde
    Permeabilization Yes - .1% triton x-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Submitted Apr 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (hek293)
    Loading amount 50 µg
    Specification hek293
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Submitted Oct 26 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (brain pericytes)
    Specification brain pericytes
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    Submitted Oct 15 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (muscle)
    Loading amount 50 µg
    Specification muscle
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Aug 27 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (dermal fibroblasts)
    Specification dermal fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton-X-100
    Blocking step fetal calf serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
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    Submitted Feb 25 2010

    1-10 of 16 Abreviews or Q&A

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