Anti-Desmin antibody (ab86083)

Overview

  • Product nameAnti-Desmin antibody
    See all Desmin primary antibodies
  • Description
    Rabbit polyclonal to Desmin
  • SpecificityReacts exclusively with Desmin.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Chicken, Hamster, Cow, Dog, Human, Zebrafish
  • Immunogen

    Full length native Desmin protein purified from a crude tissue preparation of chicken gizzard muscle by preparative gel electrophoresis.

Properties

Applications

Our Abpromise guarantee covers the use of ab86083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
  • Application notesIHC-P: 1/25 - 1/100, using avidinbiotinylated horseradish peroxidase complex (ABC) detection system.
    IHC-Fr: 1/25 - 1/100, using avidinbiotinylated horseradish peroxidase complex (ABC) detection system.
    WB: 1/100 - 1/500. Predicted molecular weight: 53 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in diseaseDefects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Anti-Desmin antibody images

    • Immunofluorescence staining images of 9 day old zebrafish embryos.
      ab86083 reacts with striated muscle of the developing myocardium. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
    • Immunofluorescence staining images of 9 day old zebrafish embryos.
      ab86083 reacts with striated muscle of the developing myocardium. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

    References for Anti-Desmin antibody (ab86083)

    ab86083 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Muscle)
    Permeabilization Yes - 0.2% Triton
    Specification Muscle
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 22 2015

    I apologize for the delay.

    Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

    ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibod...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"