Overview

  • Product nameAnti-Desmin antibody [D33]
    See all Desmin primary antibodies
  • Description
    Mouse monoclonal [D33] to Desmin
  • SpecificityThis antibody is highly reactive with desmin. On immunoblots only the 56 kD desmin band is stained. Non reactive with GFAP, Keratin, Vimentin and Neurofilament.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Hamster, Human
  • Immunogen

    Human leiomyoma

  • General notesSufficient antibody for 100-500 tests


    This antibody stains muscle cells in human tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab8470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
IHC-Fr 1/100.
IHC-P 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21699414

Target

  • FunctionDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
  • Involvement in diseaseDefects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMD1I antibody
    • CSM1 antibody
    • CSM2 antibody
    • DES antibody
    • DESM_HUMAN antibody
    • Desmin antibody
    • FLJ12025 antibody
    • FLJ39719 antibody
    • FLJ41013 antibody
    • FLJ41793 antibody
    • Intermediate filament protein antibody
    • OTTHUMP00000064865 antibody
    see all

Anti-Desmin antibody [D33] images

  • IHC image of ab8470 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8470, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Desmin antibody [D33] (ab8470)

This product has been referenced in:
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Human . Read more (PubMed: 27588108) »
  • Jia X  et al. Large cervicothoracic myxoinflammatory fibroblastic sarcoma with brachial plexus invasion: A case report and literature review. Oncol Lett 12:1717-1720 (2016). IHC-P ; Human . Read more (PubMed: 27588121) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Cytrate pH6
Permeabilization No
Specification Muscle
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Myocytes of blood vessel)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Myocytes of blood vessel
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Jul 12 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (Muscle of leg)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Cytrate pH6
Permeabilization No
Specification Muscle of leg
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Jul 12 2016

Thank you for your enquiry. Ab8470 is clone D33. I have added this information to our online datasheet. Please contact us again if you have any additional questions.

Thank you for your patience. The antibody should work very well in skeletal muscle, unless it is myopathic. With skeletal myopathy lack of demin occurs. We recommend as positive controls skeleton muscle, rhabdomyosarcomas and leiomyoma cells. We would ...

Read More

This antibody has not been tested in any other species except human. Unfortunately, it is not possible to purchase a smaller quantity for testing purposes.

Cross reactivity with rat has not yet been tested. Unfortunately we do not think that this antibody will cross react with rat.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"