Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

Overview

  • Product name
    Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
    See all Desmin primary antibodies
  • Description
    Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
  • Tested applications
    Suitable for: WB, IHC-FoFr, IHC-P, IHC-Frmore details
    Unsuitable for: ICC or ICC/IF
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake
  • Immunogen

    Full length native protein (purified) (Pig).

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Skeletal muscle.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab6322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Monoclonal anti-desmin may be used for Immunocytochemical localization of intermediate filaments of the desmin group in all types of muscle cells and to localize desmin at the periphery of z-discs.

IHC-FoFr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or ICC/IF.
  • Target

    • Function
      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in disease
      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Images

    • ab6322 at 1/100 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incuabion with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG1 was used as the secondary.

      See Abreview

    • Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322) at 1/3000 dilution + Mouse skeletal muscle tissue lysate at 30 µg

      Secondary
      HRP-conjugated Goat anti Mouse IgG polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 55 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes

      This image is courtesy of an anonymous Abreview

      Blocked with 5% Milk for 1 hour at 25°C.

      See Abreview

    • Immunohistochemical analysis of Human colorectal tissue, staining Desmin (green) with ab6322.

      Tissue was fixed with ice cold acetone for 5 minutes and blocked with 10% goat serum for 30 minutes. Samples were incubated with primary antibody (1/30) overnight at 4°C and an AlexaFluor®488®-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.
    • IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Protocols

    References

    This product has been referenced in:
    • Gertz K  et al. Partial loss of VE-cadherin improves long-term outcome and cerebral blood flow after transient brain ischemia in mice. BMC Neurol 16:144 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27538712) »
    • Park SY  et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun 7:10871 (2016). Read more (PubMed: 26972991) »

    See all 25 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (muscle)
    Permeabilization
    Yes - PBS triton0.5%
    Specification
    muscle
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 14 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Tissue lysate - whole (skeletal muscle)
    Specification
    skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Rafael Montes Nieto

    Verified customer

    Submitted Oct 31 2014

    Application
    Immunocytochemistry
    Blocking step
    FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cultured Cells (immortalized myoblasts)
    Specification
    immortalized myoblasts
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 05 2014

    I have issued a free of charge replacement with a new lot.

    Thank you for sending your protocol, which appears to be correct, and the image file. I agree that the antibody is staining something that is unlikely to be desmin. Are you interested in trying a different antibody against desmin? I see that ab15200 ha...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skeletal muscle)
    Loading amount
    30 µg
    Specification
    skeletal muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 14 2012

    Thank you for your enquiry whichi has been forwarded to the scientific support team. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed...

    Read More

    Thanks for your call today. Please see below for your testing discount codes and instructions for use. Let me know if you have any questions! DISCOUNT CODES: (1) ab15463: *** (2) ab6322: *** Expiration date: January 22, 2012 ...

    Read More

    Unfortunately, we do not sell this antibody or any other Desmin antibody in conjugated version. I do not have the information at the moment if and when a conjugated version will be added to the catalogue.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Esophagus)
    Specification
    Esophagus
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
    Username

    Dr. Vyacheslav Ogay

    Verified customer

    Submitted May 15 2007

    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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