Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
Key features and details
- Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
See all Desmin primary antibodies -
Description
Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake -
Immunogen
Full length native protein (purified) corresponding to Pig Desmin.
-
Positive control
- IHC-P: Human skeletal muscle, Mouse cardiac muscle and rat colon tissue.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
DE-U-10 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. -
Involvement in disease
Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin. -
Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 280765 Cow
- Entrez Gene: 1674 Human
- Entrez Gene: 13346 Mouse
- Entrez Gene: 64362 Rat
- Omim: 125660 Human
- SwissProt: P02542 Chicken
- SwissProt: O62654 Cow
- SwissProt: P17661 Human
see all -
Alternative names
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on mouse cardiac muscle.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on smooth muscle of rat colon.
-
IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Datasheets and documents
-
SDS download
-
Datasheet download
References (52)
ab6322 has been referenced in 52 publications.
- Jiang K et al. Diverse myopathological features in the congenital myasthenia syndrome with GFPT1 mutation. Brain Behav 12:e2469 (2022). PubMed: 34978387
- Claeyssen C et al. Global O-GlcNAcylation changes impact desmin phosphorylation and its partition toward cytoskeleton in C2C12 skeletal muscle cells differentiated into myotubes. Sci Rep 12:9831 (2022). PubMed: 35701470
- Miyata R et al. Lung fibrogenic microenvironment in mouse reconstitutes human alveolar structure and lung tumor. iScience 25:104912 (2022). PubMed: 36060050
- Koda Y et al. CD8+ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cells. Nat Commun 12:4474 (2021). PubMed: 34294714
- Wang F et al. BMSC-derived leptin and IGFBP2 promote erlotinib resistance in lung adenocarcinoma cells through IGF-1R activation in hypoxic environment. Cancer Biol Ther 21:61-71 (2020). PubMed: 31559898