Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

Overview

  • Product nameAnti-Desmin antibody [Y66] - Cytoskeleton Marker
    See all Desmin primary antibodies
  • Description
    Rabbit monoclonal [Y66] to Desmin - Cytoskeleton Marker
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IHC - Wholemount, Flow Cyt, IHC-Frmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Desmin aa 400 to the C-terminus (C terminal).

  • Epitopeab32362 reacts with an epitope located in the C terminal region of desmin.
  • Positive control
    • WB: Human skeletal muscle, fetal heart and fetal muscle tissue lysates. Mouse and rat heart tissue lysates. Guinea pig heart and muscle tissue lysates. ICC/IF: A673 cells. IHC-P: Human skeletal muscle, uterus and urinary bladder tissues. Flow Cyt: C2C12 and HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Desmin antibody (Alexa Fluor® 488) [Y66] - Cytoskeleton Marker (ab185033)

    Anti-Desmin antibody (HRP) [Y66] - Cytoskeleton Marker (ab195178)

    Anti-Desmin antibody (Alexa Fluor® 568) [Y66] - Cytoskeleton Marker (ab202503)

    Anti-Desmin antibody (Alexa Fluor® 594) [Y66] - Cytoskeleton Marker (ab203419)

    Anti-Desmin antibody (Alexa Fluor® 555) [Y66] - Cytoskeleton Marker (ab203422)

    Anti-Desmin antibody (Alexa Fluor® 647) [Y66] - Cytoskeleton Marker (ab195177)

Properties

Applications

Our Abpromise guarantee covers the use of ab32362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB 1/100000. Predicted molecular weight: 53 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

IHC - Wholemount Use at an assay dependent concentration. PubMed: 21856924
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 21824387
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • Involvement in diseaseDefects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    Anti-Desmin antibody [Y66] - Cytoskeleton Marker images

    • All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500000 dilution (purified)

      Lane 1 : Human skeletal muscle tissue lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : Human fetal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunocytochemistry/Immunofluorescence analysis of A673 cells labelling Desmin with purified ab32362 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Desmin with purified ab32362 at 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Flow Cytometry analysis of C2C12 cells labelling Desmin with purified ab32362 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500000 dilution (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Unpurified ab32362 staining Desmin (green) in Human skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - DAPI-nuclei. Red - WGA. 40X objective.

      See Abreview

    • All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/100000 dilution

      Lane 1 : Guinea pig heart tissue lysate
      Lane 2 : Guinea pig muscle tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunohistochemistry (Frozen sections) analysis of mouse skeletal muscle tissue following cardiotoxin injury, labelling Desmin with unpurified ab32362.

    • All lanes : Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362) at 1/500 dilution (unpurified)

      Lane 1 : Human skeletal muscle whole tissue lysate
      Lane 2 : Human skeletal muscle whole tissue lysate
      Lane 3 : Human skeletal muscle whole tissue lysate
      Lane 4 : Human skeletal muscle whole tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye® 680-conjugated anti-rabbit at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 53 kDa
      Observed band size : 53 kDa


      Exposure time : 50 seconds

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human uterus tissue labelling Desmin with unpurified ab32362.

    • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human urinary bladder tissue labelling Desmin with unpurified ab32362.

    • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human skeletal muscle tissue labelling Desmin with unpurified ab32362.

    • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human brain tissue. Unpurified ab32362 shows negative staining.

    • Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human tonsil tissue. Unpurified ab32362 shows negative staining.

    • Unpurified ab32362 staining Desmin in nude rat esophagheal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6. Samples were then blocked with 1% BSA for 20 minutes at 25°C and then incubated with unpurified ab32362 at a 1/400 dilution for 16 hours at 25°C. The secondary used was an undiluted goat anti-rabbit HRP conjugated polyclonal.Striated muscle cells of muscular propria are strongly positive for desmin. Smooth muscle cells and vascular smooth muscle cells in submucosal layer are also positive for desmin.

      See Abreview

    • Unpurified ab32362 staining Desmin (green) in Mouse aorta smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 10% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/150) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Blue - nuclei.

      See Abreview

    References for Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)

    This product has been referenced in:
    • Nguyen DG  et al. Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro. PLoS One 11:e0158674 (2016). Read more (PubMed: 27387377) »
    • Atkins HM  et al. Decidualization of Endometriosis in Macaques. Vet Pathol 53:1252-1258 (2016). Read more (PubMed: 27281017) »

    See all 26 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Skin)
    Permeabilization No
    Specification Skin
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Oct 26 2016

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA/PBS as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Cell (smooth muscle cell line)
    Specification smooth muscle cell line
    Permeabilization Yes - Triton X-100 0.1%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 31 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample Human Cell (Skeletal Muscle)
    Specification Skeletal Muscle
    Permeabilization No
    Fixative Methacarn
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12% Bis Tis Gel)
    Sample Human Tissue lysate - whole (Skeletal Muscle Cells)
    Specification Skeletal Muscle Cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Aorta Smooth Muscle Cells)
    Specification Aorta Smooth Muscle Cells
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Formalin
    Username

    Abcam user community

    Verified customer

    Submitted Sep 26 2013

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this c...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Primary Skeletal Myoblasts)
    Specification Primary Skeletal Myoblasts
    Fixative Formaldehyde
    Permeabilization Yes - 0,2% Triton
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Fabien LE GRAND

    Verified customer

    Submitted Nov 07 2012

    Thank you for your inquiry.

    We recommend using this antibody in FC 1"10 - 1:100 dilution. We tested permeabilized HeLa cells and the histograph can be found on the datasheet: http://www.abcam.com/Desmin-antibody-Y266-ab32362.html
    Read More

    I heard back from the lab about the last two antibodies.
    For ab32362, the immunogen sequence shares 100% homology with pig.
    For ab124687, the immunogen peptide maps between amino acids 235 - 260, on the C-terminus of human CA2 (UniProt: P0091...

    Read More

    Thank you for your inquiry.

    For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

    Read More

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