Specificityab69406 reacts with Desmocollin 2 and Desmocollin 3 human proteins, with a particularly strong reaction to Desmocollin 2. Crossreactivity with other desmocollin family members has not been observed.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 20 - 50 µg/ml.
IP: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 120 kDa, corresponding to Desmocollin 2, and a band of approximately 105 kDa, corresponding to Desmocollin 3 (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMembers of the desmocollin subfamily are calcium-dependent glycoproteins. They are part of the cadherin superfamily. These desmosomal family members, together with the desmogleins, are mainly found in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction. They are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650kb combined.
Cellular localizationCell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
References for Anti-Desmocollin 2 + 3 antibody [3G130] (ab69406)
This product has been referenced in:
Shapiro JP et al. A quantitative proteomic workflow for characterization of frozen clinical biopsies: Laser capture microdissection coupled with label-free mass spectrometry. J Proteomics : (2012).
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