Overview

  • Product nameAnti-Desmocollin 2 + 3 antibody [3G130]
  • Description
    Mouse monoclonal [3G130] to Desmocollin 2 + 3
  • Specificityab69406 reacts with Desmocollin 2 and Desmocollin 3 human proteins, with a particularly strong reaction to Desmocollin 2. Crossreactivity with other desmocollin family members has not been observed.
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Desmocollin 2 extracellular domain

  • Positive control
    • A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number3G130
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notesICC/IF: Use at a concentration of 20 - 50 µg/ml.
    IP: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 120 kDa, corresponding to Desmocollin 2, and a band of approximately 105 kDa, corresponding to Desmocollin 3 (predicted molecular weight: 100 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMembers of the desmocollin subfamily are calcium-dependent glycoproteins. They are part of the cadherin superfamily. These desmosomal family members, together with the desmogleins, are mainly found in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction. They are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650kb combined.
    • Cellular localizationCell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
    • Database links
    • Alternative names
      • ARVD11 antibody
      • CDHF2 antibody
      • CDHF3 antibody
      • Desmocollin 2 antibody
      • Desmocollin 3 antibody
      • Desmosomal glycoprotein II and III antibody
      • DG2 antibody
      • DGII/III antibody
      • DSC antibody
      • DSC1 antibody
      • DSC2 antibody
      • DSC3 antibody
      • DSC4 antibody
      see all

    References for Anti-Desmocollin 2 + 3 antibody [3G130] (ab69406)

    This product has been referenced in:
    • Shapiro JP  et al. A quantitative proteomic workflow for characterization of frozen clinical biopsies: Laser capture microdissection coupled with label-free mass spectrometry. J Proteomics : (2012). IHC . Read more (PubMed: 23022584) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab69406.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"