Anti-Desmocollin 3 antibody (ab55011)

Overview

  • Product nameAnti-Desmocollin 3 antibody
    See all Desmocollin 3 primary antibodies
  • Description
    Mouse monoclonal to Desmocollin 3
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: CKPKMGYTDI LAVDPDEPVH GAPFYFSLPN TSPEISRLWS LTKVNDTAAR LSYQKNAGFQ EYTIPITVKD RAGQAATKLL RVNLCECTHP TQCRATSRST , corresponding to amino acids 585-685 of Human Desmocollin 3

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 100 kDa.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
  • Tissue specificityEpidermis, buccal mucosa, esophagus and cervix.
  • Involvement in diseaseDefects in DSC3 are the cause of hypotrichosis and recurrent skin vesicles (HRSV) [MIM:613102]. A disorder characterized by hypotrichosis and the appearance of recurrent skin vesicle formation. Affected individuals show sparse and fragile hair on scalp, as well as absent eyebrows and eyelashes. Vesicles filled with thin, watery fluid are observed on the scalp and skin of most of the boby. Mucosal vesicles are absent.
  • Sequence similaritiesContains 5 cadherin domains.
  • DomainCalcium may be bound by the cadherin-like repeats.
  • Cellular localizationCell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin family member 3 antibody
    • CDHF3 antibody
    • desmocollin 3a/3b antibody
    • Desmocollin 4 antibody
    • Desmocollin-3 antibody
    • Desmocollin-4 antibody
    • Desmocollin3 antibody
    • Desmocollin4 antibody
    • DSC antibody
    • DSC1 antibody
    • DSC2 antibody
    • DSC3 antibody
    • DSC3_HUMAN antibody
    • DSC4 antibody
    • HT CP antibody
    • HT-CP antibody
    • HTCP antibody
    see all

Anti-Desmocollin 3 antibody images

  • Overlay histogram showing A431 cells stained with ab55011 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55011, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab55011 at 10 ug/ml staining Desmocollin 3 in human A-431 cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-Desmocollin 3 antibody (ab55011)

ab55011 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.

I am pleased to provide the following information:

The immunogen sequence for this antibody (ab55011Desmocollin 3)is provided on the datasheet:

'Recombinant fragment: CKPKMGYTDI LAVDPDEPVH GA...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"