• Product nameAnti-Desmoglein 1 antibody [27B2]
    See all Desmoglein 1 primary antibodies
  • Description
    Mouse monoclonal [27B2] to Desmoglein 1
  • SpecificityAb12077 reacts with Desmoglein 1. Does not cross-react with Desmoglein 2 or Desmoglein 3.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive



Our Abpromise guarantee covers the use of ab12077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10. Predicted molecular weight: 165 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificityEpidermis, tongue, tonsil and esophagus.
  • Involvement in diseaseDefects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
  • Sequence similaritiesContains 4 cadherin domains.
  • DomainCalcium may be bound by the cadherin-like repeats.
  • Cellular localizationCell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin family member 4 antibody
    • CDHF4 antibody
    • Desmoglein-1 antibody
    • Desmosomal glycoprotein 1 antibody
    • DG1 antibody
    • DGI antibody
    • DSG antibody
    • DSG1 antibody
    • DSG1_HUMAN antibody
    • EPKHE antibody
    • EPKHIA antibody
    • Pemphigus foliaceus antigen antibody
    • PPKS1 antibody
    • SPPK1 antibody
    see all

Anti-Desmoglein 1 antibody [27B2] images

  • ab12077 staining Desmoglein 1 in human skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.

    See Abreview

References for Anti-Desmoglein 1 antibody [27B2] (ab12077)

This product has been referenced in:
  • Tsutsumi M  et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . Read more (PubMed: 19657674) »
  • Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . Read more (PubMed: 17971852) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil epithelium)
Specification Tonsil epithelium
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Tris pH=7.4/ 1mMEDTA
Permeabilization No

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Methanol
Permeabilization Yes - 0.4% TritonX-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 08 2011

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab10277. To check the status of the order please contact our Customer...

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Thank you for your enquiry. According to the NCBI-protein site aa 664-763 are located in the cytoplasmic region of the protein. The cytoplasmic staining is therefore correct. I hope this information helps, please do not hesitate to contact us if you...

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