Overview

  • Product nameAnti-Desmoglein 1 antibody [27B2]
    See all Desmoglein 1 primary antibodies
  • Description
    Mouse monoclonal [27B2] to Desmoglein 1
  • SpecificityAb12077 reacts with Desmoglein 1. Does not cross-react with Desmoglein 2 or Desmoglein 3.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

Properties

Applications

Our Abpromise guarantee covers the use of ab12077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10. Predicted molecular weight: 165 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionComponent of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
  • Tissue specificityEpidermis, tongue, tonsil and esophagus.
  • Involvement in diseaseDefects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
  • Sequence similaritiesContains 4 cadherin domains.
  • DomainCalcium may be bound by the cadherin-like repeats.
  • Cellular localizationCell membrane. Cell junction > desmosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin family member 4 antibody
    • CDHF4 antibody
    • Desmoglein-1 antibody
    • Desmosomal glycoprotein 1 antibody
    • DG1 antibody
    • DGI antibody
    • DSG antibody
    • DSG1 antibody
    • DSG1_HUMAN antibody
    • EPKHE antibody
    • EPKHIA antibody
    • Pemphigus foliaceus antigen antibody
    • PPKS1 antibody
    • SPPK1 antibody
    see all

Anti-Desmoglein 1 antibody [27B2] images

  • ab12077 staining Desmoglein 1 in human skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.

    See Abreview

References for Anti-Desmoglein 1 antibody [27B2] (ab12077)

This product has been referenced in:
  • Tsutsumi M  et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . Read more (PubMed: 19657674) »
  • Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . Read more (PubMed: 17971852) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil epithelium)
Specification Tonsil epithelium
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Tris pH=7.4/ 1mMEDTA
Permeabilization No
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Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Methanol
Permeabilization Yes - 0.4% TritonX-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2011

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab10277. To check the status of the order please contact our Customer...

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Thank you for your enquiry. According to the NCBI-protein site aa 664-763 are located in the cytoplasmic region of the protein. The cytoplasmic staining is therefore correct. I hope this information helps, please do not hesitate to contact us if you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"