Recombinant
RabMAb

Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798)

Overview

  • Product name
    Anti-Desmoglein 1 antibody [EPR6766(B)]
    See all Desmoglein 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6766(B)] to Desmoglein 1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C-terminal of Human Desmoglein 1 (Q02413).

  • Positive control
    • Human skin lysate, Human skin tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
IHC-P 1/250 - 1/500. antigen retrieval is recommended.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
    • Tissue specificity
      Epidermis, tongue, tonsil and esophagus.
    • Involvement in disease
      Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
    • Sequence similarities
      Contains 4 cadherin domains.
    • Domain
      Calcium may be bound by the cadherin-like repeats.
    • Cellular localization
      Cell membrane. Cell junction > desmosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cadherin family member 4 antibody
      • CDHF4 antibody
      • Desmoglein-1 antibody
      • Desmosomal glycoprotein 1 antibody
      • DG1 antibody
      • DGI antibody
      • DSG antibody
      • DSG1 antibody
      • DSG1_HUMAN antibody
      • EPKHE antibody
      • EPKHIA antibody
      • Pemphigus foliaceus antigen antibody
      • PPKS1 antibody
      • SPPK1 antibody
      see all

    Images

    • Anti-Desmoglein 1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 114 kDa
    • ab124798, at a 1/250 dilution, staining Desmoglein 1 in paraffin embedded Human skin tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Quispe Calla NE  et al. Medroxyprogesterone acetate and levonorgestrel increase genital mucosal permeability and enhance susceptibility to genital herpes simplex virus type 2 infection. Mucosal Immunol 9:1571-1583 (2016). IHC-P ; Mouse . Read more (PubMed: 27007679) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Mar 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (epidermis)
    Specification
    epidermis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Epidermis)
    Specification
    Epidermis
    Permeabilization
    No
    Fixative
    Methanol
    Username

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (epidermis)
    Specification
    epidermis
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted May 29 2013

    Thank you for contacting us. Although we do not have DSG 1 and 3 antibodies that have been tested for reactivity with horse or even in all the applications you anticipate using, we do have at least one DSG1 antibody that I think will be useful.
    <...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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