Anti-Desmoglein 3/PVA antibody (ab78448)
Key features and details
- Rabbit polyclonal to Desmoglein 3/PVA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Desmoglein 3/PVA antibody
See all Desmoglein 3/PVA primary antibodies -
Description
Rabbit polyclonal to Desmoglein 3/PVA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Desmoglein 3/PVA aa 850-950 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87441) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; A431.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
Target
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Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
Epidermis, tongue, tonsil, esophagus and carcinomas. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
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Database links
- Entrez Gene: 1830 Human
- Omim: 169615 Human
- SwissProt: P32926 Human
- Unigene: 1925 Human
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Alternative names
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
see all
Images
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All lanes : Anti-Desmoglein 3/PVA antibody (ab78448) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab78448 has not yet been referenced specifically in any publications.