Anti-Desmoplakin I+II antibody [2Q400] (ab16434)

Overview

  • Product nameAnti-Desmoplakin I+II antibody [2Q400]
    See all Desmoplakin I+II primary antibodies
  • Description
    Mouse monoclonal [2Q400] to Desmoplakin I+II
  • Tested applicationsSuitable for: ICC, ICC/IF, WB, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human
  • Immunogen

    Full length protein (Cow).

  • Positive control
    • Positive cell lines: Human A432 and MCF-7; Rat MH1C1; Bovine MDBK and BMGE. Stratified epithelia, simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
  • General notesSuitable for use in the detection of primary and metastatic carcinomas.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.5% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityMonoclonal
  • Clone number2Q400
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200.
ICC/IF 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 331 kDa.
IHC-Fr 1/10.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionMajor high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
    • Tissue specificityIsoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
    • Involvement in diseaseKeratoderma, palmoplantar, striate 2
      Cardiomyopathy, dilated, with woolly hair and keratoderma
      Arrhythmogenic right ventricular dysplasia, familial, 8
      Skin fragility-woolly hair syndrome
      Epidermolysis bullosa, lethal acantholytic
      Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis
    • Sequence similaritiesBelongs to the plakin or cytolinker family.
      Contains 17 plectin repeats.
      Contains 1 SH3 domain.
      Contains 6 spectrin repeats.
    • DomainIts association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
      The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
      The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments.
    • Post-translational
      modifications
      Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
      Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).
    • Cellular localizationCell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
    • Information by UniProt
    • Database links
    • Alternative names
      • 250/210 kDa paraneoplastic pemphigus antigen antibody
      • DCWHKTA antibody
      • Desmoplakin (DPI DPII) antibody
      • Desmoplakin antibody
      • Desmoplakin I antibody
      • Desmoplakin II antibody
      • DESP_HUMAN antibody
      • DP antibody
      • DP I antibody
      • DP II antibody
      • DPI antibody
      • DPII antibody
      • DSP antibody
      • KPPS2 antibody
      • PPKS 2 antibody
      • PPKS2 antibody
      see all

    Anti-Desmoplakin I+II antibody [2Q400] images

    • ab16434 staining A431 cells by ICC/IF. Cells were methanol fixed and blocked in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/100 (PBS+10%FBS) and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/1500, was used as the secondary.

      See Abreview

    • ab16434 staining Desmoplakin I+II in rat glial cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% serum) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

      See Abreview

    References for Anti-Desmoplakin I+II antibody [2Q400] (ab16434)

    This product has been referenced in:
    • Sharma P  et al. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nat Commun 6:8391 (2015). WB, ICC/IF . Read more (PubMed: 26403541) »
    • Zarkoob H  et al. Substrate Stiffness Affects Human Keratinocyte Colony Formation. Cell Mol Bioeng 8:32-50 (2015). ICC/IF ; Human . Read more (PubMed: 26019727) »

    See all 11 Publications for this product

    Product Wall

    Thank you for your message and for providing this further information. Unfortunately, I am unsure which details about your protocol the mentioned publication explains. The expression of desmoplakin I+II in MDCK cells is of course not in question an...

    Read More
    Application Immunocytochemistry
    Sample Rat Cultured Cells (Glial Cells)
    Specification Glial Cells
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 06 2014

    My colleague told us that she did meet you and that you explainedyour disappointment about ab16434: no staining in ICC/IF after adding glycerol as recommended.

    Iam sorry to hear you are experiencing difficulties with this product. We take pro...

    Read More

    Thank you very much for your enquiry.

    Please find attached the standard protocols used with our antibodies. These protocols and further tips can also be found on our website (http://www.abcam.com/index.html?pageconfig=popular_protocols). Read More

    Vielen Dank für Ihre Anfrage und diese weiteren Informationen. Leider bin ich mir nicht ganz sicher, welche Fragen die mitgeschickte Publikation beantwortet. Die Expression von Desmoplakin I+II in MDCK-Zellen steht außer Frage und eine Untersuchung ...

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    Vielen Dank für Ihre Anfrage. Es tut mir leid, dass der Kunde Probleme mit unserem Anti-Desmoplakin I+II Antikörper ab16434 hatte. Leider ist ab16434 nicht für die Anwendung mit MDCK (dog cells) getestet und wir können nicht garantieren, dass er...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A431)
    Specification A431
    Fixative Methanol
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
    Loading amount 40 µg
    Specification MDA-MB-231 breast cancer cell line
    Gel Running Conditions Reduced Denaturing (gel 6 %)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 08 2008

    Thank you for your enquiry. I am sorry but we do not have any information where either of these antibodies have been applied in publications. Desmoplakin I+II antibody [2Q400] (ab16434) has been shown to work by Western blot, Immunohistochemist...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"