Overview

  • Product nameAnti-Desmuslin antibody [M03]
    See all Desmuslin primary antibodies
  • Description
    Mouse monoclonal [M03] to Desmuslin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 1466-1565 (VSNVEAIRSR TQEAGALGVS DRGSWRDADS RNDQAVGVSF KASAGEGDQA HREQGKEQAM FDKKVQLQRM VDQRSVISDE KKVALLYLDN EEEENDGHWF) of human Desmuslin (NP_663780) linked to a proprietary tag.

  • Positive control
    • HeLa cell lysate
  • General notes

    The recombinant fragment used as the immunogen is available as ab161606.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberM03
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab89228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 173 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionType-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.
  • Tissue specificityIsoform 2 is strongly detected in adult heart, fetal skeletal muscles and fetal heart. Isoform 1 is weakly detected in fetal heart and also in fetal skeletal muscle. Isoform 1 and isoform 2 are detected in adult bladder (at protein level). The mRNA is predominantly expressed in heart and muscle with some expression in brain which may be due to tissue-specific isoforms.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageIn lens, first detected at 16 weeks when expression is weakly and uniformly distributed. Subsequently, expression becomes much stronger in the epithelium of the anterior part at 25 weeks and later. In retina, weakly expressed at 15 weeks in the nerve fiber and ganglion cell layers (NFL and GCL). From 25 weeks onwards, much stronger expression is observed in the endfeet of Mueller cells, the NFL, and GCL, and much lower expression is observed in a minor subpopulation of cells in the inner cell layer (INL). At 30 and 36 weeks, expression remains in the neural retina, and subsequently becomes stronger in the NFL, GCL, and INL and is decreased in Mueller cells. At 36 weeks, also expressed at the external border of the outer nuclear layer (ONL) (at protein level).
  • Cellular localizationCytoplasm, cytoskeleton. Cell junction, adherens junction. There are at least two distinct SYNM subpopulations, one in which SYMN interacts with DES within the Z-lines, and another in which it interacts with both DTNA and DES at the costamere.
  • Information by UniProt
  • Database links
  • Alternative names
    • Desmuslin antibody
    • DMN antibody
    • KIAA0353 antibody
    • SYN antibody
    • SYNEM_HUMAN antibody
    • Synemin alpha antibody
    • Synemin antibody
    • Synemin beta antibody
    • Synemin, intermediate filament protein antibody
    • SYNM antibody
    see all

Anti-Desmuslin antibody [M03] images

  • Anti-Desmuslin antibody [M03] (ab89228) at 5 µg/ml + HeLa cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 173 kDa
    Observed band size : 173 kDa

References for Anti-Desmuslin antibody [M03] (ab89228)

ab89228 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"