Overview

  • Product name
  • Description
    Rabbit polyclonal to Destrin
  • Specificity
    The product cross-reacts with rat, mouse, and dog destrin but not with recombinant chicken muscle cofilin.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 153-165 of Destrin with N-terminal added lysine.

  • Positive control
    • Western blot: Whole cell extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, or dog MDCK kidney cells ICC/IF: mouse NIH/3T3 fibroblasts
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM sodium azide.
    Constituents: 0.01M PBS, 1% BSA. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1500. Detects a band of approximately 19 kDa.

Target

References

This product has been referenced in:
  • Su B  et al. Diallyl disulfide suppresses epithelial-mesenchymal transition, invasion and proliferation by downregulation of LIMK1 in gastric cancer. Oncotarget 7:10498-512 (2016). Read more (PubMed: 26871290) »
  • Luxenburg C  et al. Wdr1-mediated cell shape dynamics and cortical tension are essential for epidermal planar cell polarity. Nat Cell Biol 17:592-604 (2015). Mouse . Read more (PubMed: 25915128) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I have double-checked the information on the datasheet, as we no longer use the "immunofluorescence" application, since it is not clear what type of samples are used with that. I have updated the datasheet to reflect tha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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