Anti-Detyrosinated alpha Tubulin antibody (ab48389)


  • Product nameAnti-Detyrosinated alpha Tubulin antibody
  • Description
    Rabbit polyclonal to Detyrosinated alpha Tubulin
  • SpecificityThis product reacts specifically with human Detyrosinated alpha Tubulin.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab48389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
ICC/IF 1/200.
WB 1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human Detyrosinated alpha Tubulin peptide (ab191559).


  • RelevanceMicrotubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Alpha tubulin 1 antibody
    • TUBA1 antibody
    • TUBA2 antibody
    • TUBA3C antibody
    • TUBA4A antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 1 testis specific antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 4a antibody
    see all

Anti-Detyrosinated alpha Tubulin antibody images

  • All lanes : Anti-Detyrosinated alpha Tubulin antibody (ab48389) at 1/500 dilution

    Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Lanes 1 - 3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab48389 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48389, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Detyrosinated alpha Tubulin antibody (ab48389)

This product has been referenced in:
  • Gerdes JM  et al. Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents. Nat Commun 5:5308 (2014). Read more (PubMed: 25374274) »
  • Veland IR  et al. Inversin/Nephrocystin-2 is required for fibroblast polarity and directional cell migration. PLoS One 8:e60193 (2013). ICC/IF ; Mouse . Read more (PubMed: 23593172) »

See all 6 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Zebrafish Embryo (whole embryos caudal fin imaged)
Specification whole embryos caudal fin imaged

Abcam user community

Verified customer

Submitted May 12 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (swine testicle (ST))
Permeabilization Yes - triton
Specification swine testicle (ST)
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Sep 11 2015

Application Immunocytochemistry
Sample Rat Cultured Cells (Sertoli cells)
Permeabilization Yes - Triton
Specification Sertoli cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative Methanol

Dr. Nan Li

Verified customer

Submitted Sep 09 2015

Application Western blot
Sample Rat Cell lysate - whole cell (Sertoli cells)
Gel Running Conditions Reduced Denaturing (12.5% gel)
Loading amount 20 µg
Specification Sertoli cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Nan Li

Verified customer

Submitted Sep 09 2015

Thank you for your enquiry regarding ab48389.

This product is whole serum and not affinity purified so the concentration is not determined. You can find some more information about the concentration of the different purifications at this site...

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