DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
DNA fragmentation factor, 40 kD beta subunit antibody
DNA fragmentation factor, 40 kD, beta polypeptide antibody
DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
Western blot - DFFB antibody (ab69438)
All lanes : Anti-DFFB antibody (ab69438) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa Observed band size: 39 kDa Additional bands at: 15 kDa (possible isoform), 30 kDa (possible isoform), 75 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3 and 4) of DNA fragmentation factor subunit beta (DFFB). The predicted molecular weights of isoforms (1,2,3 and 4) are 39 kDa, 30 kDa, 12 kDa and 11 kDa respectively.
ICC/IF image of ab69438 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69438, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and MCF-7 cells at 5µg/ml.