Anti-DFFB/CAD antibody (ab8401)
Key features and details
- Rabbit polyclonal to DFFB/CAD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-DFFB/CAD antibody
See all DFFB/CAD primary antibodies -
Description
Rabbit polyclonal to DFFB/CAD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Murine kidney tissue lysate. A 40 kDaband should be detected.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
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IHC-P |
Use a concentration of 2 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
IHC-P
Use a concentration of 2 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. -
Sequence similarities
Contains 1 CIDE-N domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 13368 Mouse
- SwissProt: O54788 Mouse
- Unigene: 388918 Mouse
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Alternative names
- CAD antibody
- Caspase activated deoxyribonuclease antibody
- Caspase activated DNase antibody
see all
Images
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Anti-DFFB/CAD antibody (ab8401) at 2 µg/ml + murine kidney tissue lysate
Predicted band size: 39 kDa -
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling DFFB/CAD with Anti-DFFB/CAD antibody (ab8401) at 5μg/ml.
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ab8401 at 2µg/ml staining DFFB/CAD in mouse kidney tissue by IHC
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Immunofluorescence of DFFB/CAD in Mouse Kidney cells using ab8401 at 10 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab8401 has not yet been referenced specifically in any publications.