Overview

  • Product name
  • Description
    Rabbit polyclonal to DFFB
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    QKPKSVKLRALRSPRKC

    , corresponding to amino acids 3/18 of Human DFFB (Peptide available as ab8440.)

  • Positive control
    • K562 or Jurkat whole cell lysate A 40 kDa band can be detected.

Properties

Applications

Our Abpromise guarantee covers the use of ab8408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 40 kDa.Can be blocked with Human DFFB peptide (ab8440).

Target

  • Function
    Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
  • Sequence similarities
    Contains 1 CIDE-N domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD antibody
    • Caspase activated deoxyribonuclease antibody
    • Caspase activated DNase antibody
    • Caspase activated nuclease antibody
    • Caspase-activated deoxyribonuclease antibody
    • Caspase-activated DNase antibody
    • Caspase-activated nuclease antibody
    • CPAN antibody
    • Deoxyribonuclease III, caspase activated antibody
    • DFF 40 antibody
    • DFF-40 antibody
    • DFF2 antibody
    • DFF40 antibody
    • Dffb antibody
    • DFFB_HUMAN antibody
    • Didff antibody
    • DNA fragmentation factor 40 kDa subunit antibody
    • DNA fragmentation factor subunit beta antibody
    • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
    • DNA fragmentation factor, 40 kD beta subunit antibody
    • DNA fragmentation factor, 40 kD, beta polypeptide antibody
    • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
    • OTTHUMP00000003633 antibody
    see all

Images

  • All lanes : Anti-DFFB antibody (ab8408) at 1/500 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : K562 whole cell lysate


    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab8408 at 5µg/ml staining DFFBin Jurkat cells by ICC/IF

References

ab8408 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab8408.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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