Overview

  • Product nameAnti-DFFB antibody - N-terminal
    See all DFFB primary antibodies
  • Description
    Rabbit polyclonal to DFFB - N-terminal
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    QKPKSVKLRALRSPRK

    , corresponding to amino acids 3-18 of Human DFFB

  • Positive control
    • K562 whole cell lysate. Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human DFFB peptide (ab8440).

Target

  • FunctionNuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
  • Sequence similaritiesContains 1 CIDE-N domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD antibody
    • Caspase activated deoxyribonuclease antibody
    • Caspase activated DNase antibody
    • Caspase activated nuclease antibody
    • Caspase-activated deoxyribonuclease antibody
    • Caspase-activated DNase antibody
    • Caspase-activated nuclease antibody
    • CPAN antibody
    • Deoxyribonuclease III, caspase activated antibody
    • DFF 40 antibody
    • DFF-40 antibody
    • DFF2 antibody
    • DFF40 antibody
    • Dffb antibody
    • DFFB_HUMAN antibody
    • Didff antibody
    • DNA fragmentation factor 40 kDa subunit antibody
    • DNA fragmentation factor subunit beta antibody
    • DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody
    • DNA fragmentation factor, 40 kD beta subunit antibody
    • DNA fragmentation factor, 40 kD, beta polypeptide antibody
    • DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody
    • OTTHUMP00000003633 antibody
    see all

Anti-DFFB antibody - N-terminal images

  • All lanes : Anti-DFFB antibody - N-terminal (ab62150) at 1/500 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : K562 whole cell lysate


    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DFFB antibody - N-terminal (ab62150)

ab62150 has not yet been referenced specifically in any publications.

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