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Synthetic peptide within Human DFNA5/GSDME aa 66-95 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60443
Our Abpromise guarantee covers the use of ab175614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 55 kDa.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast carcinoma tissue, labeling DFNA5 using ab175614 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometry analysis of MDA-MB468 cells labeling DFNA5 (green, bottom histogram), using ab175614 at a 1/10 dilution, and negative control cells (blue, top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175614 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"