Anti-DGAT2 antibody (ab59493)
Key features and details
- Goat polyclonal to DGAT2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DGAT2 antibody
See all DGAT2 primary antibodies -
Description
Goat polyclonal to DGAT2 -
Host species
Goat -
Specificity
This antibody is not expected to cross-react with other DGAT members. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Synthetic peptide:
C-KLEHPTQQDIDLYH
, corresponding to C terminal amino acids 346-359 of Human DGAT2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Target
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Function
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. -
Tissue specificity
Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin. -
Pathway
Glycerolipid metabolism; triacylglycerol biosynthesis. -
Sequence similarities
Belongs to the diacylglycerol acyltransferase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 404129 Cow
- Entrez Gene: 84649 Human
- Entrez Gene: 67800 Mouse
- Entrez Gene: 252900 Rat
- SwissProt: Q70VZ8 Cow
- SwissProt: Q96PD7 Human
- SwissProt: Q9DCV3 Mouse
- SwissProt: Q5FVP8 Rat
see all -
Alternative names
- DGAT2 antibody
- DGAT2_HUMAN antibody
- Diacylglycerol O acyltransferase like protein 2 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab59493 has been referenced in 9 publications.
- Zhang JF et al. Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells. Animals (Basel) 12:N/A (2022). PubMed: 35883393
- O'Brien PD et al. Integrated lipidomic and transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes. Dis Model Mech 13:N/A (2020). PubMed: 31822493
- do Carmo JM et al. Impact of leptin deficiency compared with neuronal-specific leptin receptor deletion on cardiometabolic regulation. Am J Physiol Regul Integr Comp Physiol 317:R552-R562 (2019). PubMed: 31411897
- Jia H et al. Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. J Dairy Sci 102:833-845 (2019). PubMed: 30415861
- Howie D et al. A Novel Role for Triglyceride Metabolism in Foxp3 Expression. Front Immunol 10:1860 (2019). PubMed: 31456800
- Balaban S et al. Extracellular Fatty Acids Are the Major Contributor to Lipid Synthesis in Prostate Cancer. Mol Cancer Res 17:949-962 (2019). PubMed: 30647103
- Nagarajan SR et al. Lipid and glucose metabolism in hepatocyte cell lines and primary mouse hepatocytes: a comprehensive resource for in vitro studies of hepatic metabolism. Am J Physiol Endocrinol Metab 316:E578-E589 (2019). PubMed: 30694691
- Trogen G et al. Transgenic overexpression of CTRP3 prevents alcohol-induced hepatic triglyceride accumulation. Am J Physiol Endocrinol Metab 315:E949-E960 (2018). PubMed: 29763374
- Alonso C et al. Metabolomic Identification of Subtypes of Nonalcoholic Steatohepatitis. Gastroenterology 152:1449-1461.e7 (2017). PubMed: 28132890