Overview

  • Product nameAnti-DGAT2 antibody
    See all DGAT2 primary antibodies
  • Description
    Goat polyclonal to DGAT2
  • SpecificityThis antibody is not expected to cross-react with other DGAT members.
  • Tested applicationsSuitable for: Sandwich ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-KLEHPTQQDIDLYH

    , corresponding to C terminal amino acids 346-359 of Human DGAT2

Properties

Applications

Our Abpromise guarantee covers the use of ab59493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml.

Target

  • FunctionEssential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • Tissue specificityPredominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • PathwayGlycerolipid metabolism; triacylglycerol biosynthesis.
  • Sequence similaritiesBelongs to the diacylglycerol acyltransferase family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

Anti-DGAT2 antibody images

  • ab59493 at 3ug/ml staining DGAT2 in human liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval by microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.

  • All lanes : Anti-DGAT2 antibody (ab59493) at 0.3 µg/ml

    Lane 1 : Cell lysates prepared from DGAT2 transiently transfected HEK293 cells.
    Lane 2 : Cell lysates prepared from mock transfected HEK293 cells.
    Lane 3 : Tissue lysates prepared from human liver tissue.


    Observed band size : 45,60 kDa (why is the actual band size different from the predicted?)
  • Anti-DGAT2 antibody (ab59493) at 0.5 µg/ml + Human Liver lysate at 35 µg

References for Anti-DGAT2 antibody (ab59493)

ab59493 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input 500 µg
Specification Hepa1-6 hepatoma cells
Immuno-precipitation step Protein A
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Submitted Sep 05 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount 40 µg
Specification Hepa1-6 hepatoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Apr 17 2012

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse muscle)
Loading amount 40 µg
Specification Mouse muscle
Gel Running Conditions Non-reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"