• Product nameAnti-DGAT2 antibody
    See all DGAT2 primary antibodies
  • Description
    Rabbit polyclonal to DGAT2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 153-362 of Human DGAT2 (NP_115953).

  • Positive control
    • WB: Raji whole cell lysate IHC-P: A549 xenograft



Our Abpromise guarantee covers the use of ab96094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/500.


  • FunctionEssential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation.
  • Tissue specificityPredominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.
  • PathwayGlycerolipid metabolism; triacylglycerol biosynthesis.
  • Sequence similaritiesBelongs to the diacylglycerol acyltransferase family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGAT2 antibody
    • DGAT2_HUMAN antibody
    • Diacylglycerol O acyltransferase like protein 2 antibody
    • Diacylglycerol O-acyltransferase 2 antibody
    • Diacylglycerol O-acyltransferase homolog 2 (mouse) antibody
    • Diacylglycerol O-acyltransferase homolog 2 antibody
    • Diacylglycerol O-acyltransferase-like protein 2 isoform 1 antibody
    • Diglyceride acyltransferase 2 antibody
    • DKFZp686A15125 antibody
    • GS1999full antibody
    • HMFN1045 antibody
    see all

Anti-DGAT2 antibody images

  • Anti-DGAT2 antibody (ab96094) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 44 kDa
    7.5% SDS-PAGE
  • ab96094, at 1/100 dilution, staining DGAT2 in paraffin embedded A549 xenograft by Immunohistochemistry.
  • ICC/IF image of ab96094 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96094, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DGAT2 antibody (ab96094)

This product has been referenced in:
  • Lolicato F  et al. The cumulus cell layer protects the bovine maturing oocyte against fatty acid-induced lipotoxicity. Biol Reprod 92:16 (2015). Cow . Read more (PubMed: 25297544) »
  • Lee TI  et al. Cardiac metabolism, inflammation, and peroxisome proliferator-activated receptors modulated by 1,25-dihydroxyvitamin D3 in diabetic rats. Int J Cardiol 176:151-7 (2014). Rat . Read more (PubMed: 25062566) »

See all 3 Publications for this product

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Thank you for contacting us. I am sorry to read that your results were not as expected.

I will be happy to discuss a refund or make suggestions for a replacement but first, could you please send me a few details of the western blot samples, ...

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Thank you for your reply. You can go ahead and order the antibody, and redeem this code with your next order after submitting your Abreview for ab96094. DISCOUNT CODE: *** Expiration date: April 1, 2012 I am very pleased to hear you would like to test ...

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Thank you for your reply. Since ab96094 has not been tested in mouse or rat, I can issue you the testing code as soon as you are ready. All of the information that we have about this antibody is on the datasheet, so based on this information it seems t...

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Thank you for contacting us with your enquiry. We do have a testing discount program for trying an antibody in an untested species or application. We have tested ab96094 for immunofluorescent staining of human cell lines- will you be using the ...

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