For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to DGCR14 - C-terminal
Predicted to work with:
Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human DGCR14 aa 426-476 (C terminal). The exact sequence is proprietary. (NP_073210.1).
HeLa, 293T, NIH3T3 and TCMK-1 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab194883 was affinity purified using an epitope specific to DGCR14 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2500. Predicted molecular weight: 53 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in pre-mRNA splicing.
Highly expressed in heart, brain and skeletal muscle. Detected at low levels in placenta.
Belongs to the DGCR14 family.
Information by UniProt
Western blot - Anti-DGCR14 antibody - C-terminal (ab194883)
All lanes :
Anti-DGCR14 antibody - C-terminal (ab194883) at 1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lane 4 :
TCMK-1 whole cell lysate
Lane 5 :
NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique
Predicted band size :
Exposure time :
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-DGCR14 antibody - C-terminal (ab194883)
Detection of DGCR14 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab194883 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"