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Rabbit polyclonal to DGCR14 - C-terminal
Predicted to work with:
Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human DGCR14 aa 426-476 (C terminal). The exact sequence is proprietary. (NP_073210.1).
HeLa, 293T, NIH3T3 and TCMK-1 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab194883 was affinity purified using an epitope specific to DGCR14 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2500. Predicted molecular weight: 53 kDa.
Use at 2-10 µg/mg of lysate.
May be involved in pre-mRNA splicing.
Highly expressed in heart, brain and skeletal muscle. Detected at low levels in placenta.
Belongs to the DGCR14 family.
Information by UniProt
Western blot - Anti-DGCR14 antibody - C-terminal (ab194883)
All lanes :
Anti-DGCR14 antibody - C-terminal (ab194883) at 1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lane 4 :
TCMK-1 whole cell lysate
Lane 5 :
NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size:
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-DGCR14 antibody - C-terminal (ab194883)
Detection of DGCR14 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab194883 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"