Anti-DGCR14 antibody - C-terminal (ab194883)

Overview

  • Product name
    Anti-DGCR14 antibody - C-terminal
    See all DGCR14 primary antibodies
  • Description
    Rabbit polyclonal to DGCR14 - C-terminal
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DGCR14 aa 426-476 (C terminal). The exact sequence is proprietary. (NP_073210.1).
    Sequence:

    THLKTPASGLQTPTSTPAPGSATRTPLTQDPASITDNLLQLPARRKASDF F


    Database link: Q96DF8

  • Positive control
    • HeLa, 293T, NIH3T3 and TCMK-1 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab194883 was affinity purified using an epitope specific to DGCR14 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 53 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-DGCR14 antibody - C-terminal (ab194883) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 53 kDa


    Exposure time : 3 minutes

    Lysates prepared using NETN lysis buffer.

  • Detection of DGCR14 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab194883 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab194883 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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