Overview

  • Product name
  • Description
    Mouse polyclonal to DGKA
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (NP_001336.2)

  • Positive control
    • Human spleen lysate; Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.
  • Tissue specificity
    Lymphocytes and oligodendroglial cells.
  • Sequence similarities
    Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 EF-hand domains.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80 kDa diacylglycerol kinase antibody
    • DAG kinase alpha antibody
    • DAGK antibody
    • DAGK1 antibody
    • DGK alpha antibody
    • DGK-alpha antibody
    • dgkA antibody
    • DGKA_HUMAN antibody
    • Diacylglycerol kinase alpha antibody
    • Diacylglycerol kinase, alpha 80kDa antibody
    • Diglyceride kinase alpha antibody
    • MGC12821 antibody
    • MGC42356 antibody
    • OTTHUMP00000242836 antibody
    • OTTHUMP00000242955 antibody
    • OTTHUMP00000244046 antibody
    see all

Anti-DGKA antibody images

  • Anti-DGKA antibody (ab88672) at 1 µg/ml + Human spleen lysate at 50 µg

    Predicted band size : 83 kDa
  • All lanes : Anti-DGKA antibody (ab88672) at 1 µg/ml

    Lane 1 : DGKA transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence staining of DGKA in HeLa cells using ab88672 at 10 µg/ml.

References for Anti-DGKA antibody (ab88672)

This product has been referenced in:
  • Lupino E  et al. I?B kinase ß is required for activation of NF-?B and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells. J Immunol 188:2545-55 (2012). Human . Read more (PubMed: 22331067) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.

Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

To our knowledge, ab88672 has not been tested in baboon. However, the human DGKA protein sequ...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Recombinant protein (lysate of Cos'7 overexpressing GFP-human, mouse,)
Loading amount
75 µg
Specification
lysate of Cos'7 overexpressing GFP-human, mouse,
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Y Shirai

Verified customer

Submitted Aug 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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