Overview

  • Product name
  • Description
    Rabbit polyclonal to DGKE
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) derived from an internal region of DGKE

  • Positive control
    • Extracts from K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

Target

  • Function
    Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
  • Tissue specificity
    Expressed predominantly in testis.
  • Sequence similarities
    Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHUS7 antibody
    • DAG kinase epsilon antibody
    • DAGK 5 antibody
    • DAGK 6 antibody
    • DAGK5 antibody
    • DAGK6 antibody
    • DGK antibody
    • DGK epsilon antibody
    • DGK-epsilon antibody
    • DGKE antibody
    • DGKE_HUMAN antibody
    • Diacylglycerol kinase epsilon 64kDa antibody
    • Diacylglycerol kinase epsilon antibody
    • Diglyceride kinase epsilon antibody
    • NPHS7 antibody
    see all

Images

  • All lanes : Anti-DGKE antibody (ab69760) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

The blocking peptide for ab69760 anti DGKE antibody is now listed on our catalog. The product id is ab156171 DGKE peptide. The product page may be found at: http://www.abcam.com/index.html?datasheet=156171

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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