Overview

  • Product name
  • Description
    Rabbit polyclonal to DGKE
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) derived from an internal region of DGKE

  • Positive control
    • Extracts from K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

Target

  • Function
    Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
  • Tissue specificity
    Expressed predominantly in testis.
  • Sequence similarities
    Belongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHUS7 antibody
    • DAG kinase epsilon antibody
    • DAGK 5 antibody
    • DAGK 6 antibody
    • DAGK5 antibody
    • DAGK6 antibody
    • DGK antibody
    • DGK epsilon antibody
    • DGK-epsilon antibody
    • DGKE antibody
    • DGKE_HUMAN antibody
    • Diacylglycerol kinase epsilon 64kDa antibody
    • Diacylglycerol kinase epsilon antibody
    • Diglyceride kinase epsilon antibody
    • NPHS7 antibody
    see all

Anti-DGKE antibody images

  • All lanes : Anti-DGKE antibody (ab69760) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DGKE antibody (ab69760)

This product has been referenced in:

See 1 Publication for this product

Product Wall

The blocking peptide for ab69760 anti DGKE antibody is now listed on our catalog. The product id is ab156171 DGKE peptide. The product page may be found at: http://www.abcam.com/index.html?datasheet=156171

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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