The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionHighly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Tissue specificityExpressed predominantly in testis.
Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers.