Overview

  • Product nameAnti-DGKE antibody
    See all DGKE primary antibodies
  • Description
    Rabbit polyclonal to DGKE
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (EAERRPAPGS PSEGLFADGH LILWTLCSVL LPVFITFWCS LQRSRRQLHR) of Human DGKE (NP_003638)

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHighly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
  • Tissue specificityExpressed predominantly in testis.
  • Sequence similaritiesBelongs to the eukaryotic diacylglycerol kinase family.
    Contains 1 DAGKc domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAG kinase epsilon antibody
    • DAGK 5 antibody
    • DAGK 6 antibody
    • DAGK5 antibody
    • DAGK6 antibody
    • DGK antibody
    • DGK epsilon antibody
    • DGK-epsilon antibody
    • DGKE antibody
    • DGKE_HUMAN antibody
    • Diacylglycerol kinase epsilon 64kDa antibody
    • Diacylglycerol kinase epsilon antibody
    • Diglyceride kinase epsilon antibody
    see all

Anti-DGKE antibody images

  • Anti-DGKE antibody (ab84331) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit Ig at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-DGKE antibody (ab84331)

ab84331 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab84331.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"