Overview

  • Product nameAnti-DGKH antibody
    See all DGKH primary antibodies
  • Description
    Rabbit polyclonal to DGKH
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region of Human DGKH

  • Positive control
    • Fetal heart lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab80693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/1562500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).Can be blocked with Human DGKH peptide (ab99354). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/500.

Target

  • RelevanceThis gene encodes a member of the diacylglycerol kinase (DGK) enzyme family of proteins, specifically the type II DGK subfamily. Members of this family are involved in regulating the intracellular concentrations of diacylglycerol and phosphatidic acid. Two transcript variants encoding distinct isoforms have been identified for this gene.
  • Cellular localizationCytoplasm. Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association.
  • Database links
  • Alternative names
    • DAG kinase eta antibody
    • DGK eta antibody
    • Diacylglycerol kinase eta antibody
    • Diglyceride kinase eta antibody

Anti-DGKH antibody images

  • Sample Type : Adult mouse cortex. Primary Antibody Dilution : 1:500. Secondary Antibody : Anti-rabbit-Cy3. Secondary Antibody Dilution : 1:1000. Color/Signal Descriptions : Red: DGKH Cyan: Nissl (Neurons). Gene Name : DGKH.
  • Anti-DGKH antibody (ab80693) at 1 µg/ml + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Gel concentration 6-18%

References for Anti-DGKH antibody (ab80693)

This product has been referenced in:
  • Martinez K  et al. Diacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes. J Lipid Res 54:662-70 (2013). Human . Read more (PubMed: 23264678) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab80693.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"