Overview

  • Product nameAnti-DGKI antibody
    See all DGKI primary antibodies
  • Description
    Rabbit polyclonal to DGKI
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C terminal region of human DGKI.

  • Positive control
    • Extracts from 293 cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 117 kDa).

Target

  • RelevanceThis gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
  • Cellular localizationCytoplasm. Nucleus.
  • Database links
  • Alternative names
    • DAG kinase iota antibody
    • DGK iota antibody
    • DGKiota antibody
    • Diacylglycerol kinase iota antibody
    • Diglyceride kinase iota antibody
    see all

Anti-DGKI antibody images

  • Immunofluorescence analysis of HeLa cells, using DGKI antibody (ab64975) at 1/500 dilution. Left: no immunising peptide. Right: with immunising peptide.
  • All lanes : Anti-DGKI antibody (ab64975) at 1/500 dilution

    Lane 1 : Extracts from 293 cells with no immunising peptide
    Lane 2 : Extracts from 293 cells with immunising peptide


    Predicted band size : 117 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)

References for Anti-DGKI antibody (ab64975)

ab64975 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"