• Product name
  • Description
    Rabbit polyclonal to DGKZ
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1067-1117 of Human DGKZ (NP_001099010.1)

  • Positive control
    • HeLa and 293T whole cell lysate



Our Abpromise guarantee covers the use of ab105195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 124 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
    • Tissue specificity
      Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 1 is predominantly expressed in muscle.
    • Sequence similarities
      Belongs to the eukaryotic diacylglycerol kinase family.
      Contains 2 ANK repeats.
      Contains 1 DAGKc domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
    • Post-translational
      Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.
    • Cellular localization
      Cytoplasm. Nucleus. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAG kinase zeta antibody
      • DAGK5 antibody
      • DAGK5 PEN antibody
      • DAGK6 antibody
      • DGK ZETA antibody
      • DGK-zeta antibody
      • Dgkz antibody
      • DGKZ_HUMAN antibody
      • Diacylglycerol kinase zeta antibody
      • Diglyceride kinase zeta antibody
      • hDGKzeta antibody
      see all


    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DGKZ with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling DGKZ with ab105195 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-DGKZ antibody (ab105195) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 124 kDa

      Exposure time : 30 seconds


    This product has been referenced in:
    • Torres-Ayuso P  et al. Diacylglycerol kinase-? regulates mTORC1 and lipogenic metabolism in cancer cells through SREBP-1. Oncogenesis 4:e164 (2015). Read more (PubMed: 26302180) »
    • Gharbi SI  et al. Transient PKCa shuttling to the immunological synapse is governed by DGK? and regulates L-selectin shedding. J Cell Sci 126:2176-86 (2013). Read more (PubMed: 23525016) »

    See all 2 Publications for this product

    Customer reviews and Q&As

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    The predicted band size according to Swissprot is 124kDa. http://www.uniprot.org/uniprot/Q13574

    The observed band size will be slightly different due to post translational modifications and processing o...

    Read More


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