Overview

  • Product name
    Anti-DHEA 3-Sulfate antibody
    See all DHEA 3-Sulfate primary antibodies
  • Description
    Sheep polyclonal to DHEA 3-Sulfate
  • Specificity
    Cross reactivity : DHEAS 100% DHEA 69% 5-alpha-Androstendione 5.3% 5 Androstendione 3.6% 4 Androstendione 2.3% Androstendione 3.0% Testosterone <0.3% Androstendiol <0.4%
  • Tested applications
    Suitable for: RIAmore details
  • Immunogen

    DHEA 3-Sulfate conjugated to HMS-BSA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Blue Sepharose chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA
  • Application notes
    RIA: 100 µl/test (3H).

    Assay buffer : 0.01 M PBS, pH 7.4 with 0.1% gelatin and 0.1% thiomersal.

    Add 500 µl buffer and divide into 100 µl aliquots for storage at -20ºC. When required, dilute each aliquot to 10 ml using assay buffer. Use 100 µl/test.

    Use Dextran coated charcoal to separate.

    The antiserum is sufficiently specific and does not require pre-assay chromatographic purification. No extraction of the sample is required.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      DHEA (Dehydroepiandrosterone) is an endogenous steroid hormone made in the human body, and secreted by the adrenal gland. DHEA serves as precursor to male and female sex hormones (androgens and estrogens). Dehydroepiandrosterone sulfate (DHEAS) is the sulfated version of DHEA. This conversion is reversibly catalysed by sulfotransferase (SULT2A1) primarily in the adrenals, the liver, and small intestines. In blood, most DHEA is found as DHEAS with levels that are about 300 times higher than free DHEA. While DHEA levels reach their peak in the early morning hours, DHEAS levels show no diurnal variation. DHEAS is biologically active only after its sulfate group has been split and it becomes DHEA again. Measurement of DHEAS is preferable to DHEA as levels are more stable.
    • Cellular localization
      Secreted
    • Alternative names
      • Dehydroepiandrosterone sulfate antibody

    References for Anti-DHEA 3-Sulfate antibody (ab36365)

    ab36365 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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