Overview

  • Product name
    DHEA-S ELISA Kit
    See all DHEA-S kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 14 <=7.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9 <=14.9%
  • Sample type
    Saliva
  • Assay type
    Competitive
  • Sensitivity
    0.05 ng/ml
  • Range
    0.2 ng/ml - 12 ng/ml
  • Recovery

    108.86 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Dehydroepiandrosterone sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA-S in saliva.

    A 96-well plate has been precoated with anti-DHEA-S antibodies. Samples and the DHEA-S-HRP conjugate are added to the wells, where DHEA-S in the sample competes with the added DHEA-S-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA-S in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    50X Washing Solution 1 x 20ml
    Anti-DHEA-S IgG Coated Microplate (12 x 8 wells) 1 unit
    Cover Foil 1 unit
    DHEA-S High Control 1 x 1ml
    DHEA-S HRP Conjugate 1 x 1ml
    DHEA-S Low Control 1 x 1ml
    DHEA-S Standard 0 - 0 ng/mL 1 x 1ml
    DHEA-S Standard 1 - 0.2 ng/mL 1 x 1ml
    DHEA-S Standard 2 - 1.0 ng/mL 1 x 1ml
    DHEA-S Standard 3 - 3.0 ng/mL 1 x 1ml
    DHEA-S Standard 4 - 12.0 ng/mL 1 x 1ml
    Incubation Buffer 1 x 30ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab178642 has not yet been referenced specifically in any publications.

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