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Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.
A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|10X Wash Solution||1 x 50ml|
|Serum Diluent||1 x 100ml|
|Anti-DHEA sulfate antibody Coated Microplate (12 x 8 wells)||1 unit|
|Control A||1 x 1ml|
|Control B||1 x 1ml|
|Cover foils||1 unit|
|DHEA sulfate Standard 0 – 0 µg/mL||1 x 1ml|
|DHEA sulfate Standard 1 – 0.1 µg/mL||1 x 1ml|
|DHEA sulfate Standard 2 – 0.4 µg/mL||1 x 1ml|
|DHEA sulfate Standard 3 – 1.0 µg/mL||1 x 1ml|
|DHEA sulfate Standard 4 – 4.0 µg/mL||1 x 1ml|
|DHEA sulfate Standard 5 – 10.0 µg/mL||1 x 1ml|
|DHEA sulfate-HRP Conjugate||1 x 12ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|