Overview

  • Product name
    DHEA sulfate (DHEA-S) ELISA Kit
    See all DHEA-S kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Control 64 < 7.9%
    Inter-assay
    Sample n Mean SD CV%
    Control 48 < 10.4%
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.04 ng/ml
  • Range
    0.1 µg/ml - 10 µg/ml
  • Recovery

    = 96.71 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DHEA sulfate (DHEA-S) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of DHEA sulfate in plasma and serum.

     

    A 96-well plate has been precoated with anti-DHEA sulphate antibodies. Samples and the DHEA sulfate-HRP conjugate are added to the wells, where DHEA sulfate in the sample competes with the added DHEA sulfate-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of DHEA sulfate in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Serum Diluent 1 x 100ml
    Anti-DHEA sulfate antibody Coated Microplate (12 x 8 wells) 1 unit
    Control A 1 x 1ml
    Control B 1 x 1ml
    Cover foils 1 unit
    DHEA sulfate Standard 0 – 0 µg/mL 1 x 1ml
    DHEA sulfate Standard 1 – 0.1 µg/mL 1 x 1ml
    DHEA sulfate Standard 2 – 0.4 µg/mL 1 x 1ml
    DHEA sulfate Standard 3 – 1.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 4 – 4.0 µg/mL 1 x 1ml
    DHEA sulfate Standard 5 – 10.0 µg/mL 1 x 1ml
    DHEA sulfate-HRP Conjugate 1 x 12ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108669

Protocols

References

This product has been referenced in:
  • Grimby-Ekman A  et al. Different DHEA-S Levels and Response Patterns in Individuals with Chronic Neck Pain, Compared with a Pain Free Group-a Pilot Study. Pain Med N/A:N/A (2016). Competitive ELISA ; Human . Read more (PubMed: 27498549) »

See 1 Publication for this product

Customer reviews and Q&As

The standard range of 0.1 - 10 ug/ml takes into account the 1/50 sample dilution that is done, so you do not need to multiply your end measurement by 50.

DHEA-S and DHEA are very similar molecules: our antibody has more affinity for DHEA compared to DHEA-S (10 % higher). You have to consider that in human blood the quantity of DHEA is 1.000 times lower than DHEA-S (from clinical point of view the elevat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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