Overview

  • Product name
  • Description
    Rabbit polyclonal to DHFRL1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DHFRL1 aa 44-74 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q86XF0

  • Positive control
    • 293, HeLa and K562 cell line lysates. Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab171837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 22 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    May play a role in folate metabolism. Belongs to the dihydrofolate reductase family.
  • Cellular localization
    Mitochondrion inner membrane
  • Database links
  • Alternative names
    • DHFRP4 antibody
    • Dihydrofolate reductase like 1 antibody
    • Dihydrofolate reductase pseudogene 4 antibody

Images

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human liver tissue labeling DHFRL1 using ab171837 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-DHFRL1 antibody (ab171837) at 1/100 dilution

    Lane 1 : 293 cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : K562 cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 22 kDa

References

ab171837 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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