Overview

  • Product nameAnti-Dhh antibody [RM0086-6A12]
    See all Dhh primary antibodies
  • Description
    Rat monoclonal [RM0086-6A12] to Dhh
  • Specificityab86061 detects Mouse Dhh N terminal polypeptide, but not C terminal polypeptide.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse recombinant Dhh N-terminal fragment

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThe IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and filter sterilized through a 0.2 µm filter.
  • ClonalityMonoclonal
  • Clone numberRM0086-6A12
  • IsotypeIgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Predicted molecular weight: Unprocessed precursor, 44 kDa; Dhh protein N-product, 20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIntercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
    • Involvement in diseaseDefects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
      Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.
    • Sequence similaritiesBelongs to the hedgehog family.
    • Post-translational
      modifications
      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    • Cellular localizationSecreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • C78960 antibody
      • Desert hedgehog (Drosophila) homolog antibody
      • Desert hedgehog antibody
      • Desert hedgehog homolog (Drosophila) antibody
      • Desert hedgehog protein C-product antibody
      • Desert hedgehog protein precursor antibody
      • DHH antibody
      • DHH_HUMAN antibody
      • GDXYM antibody
      • HHG-3 antibody
      • Hira antibody
      • SRXY7 antibody
      see all

    References for Anti-Dhh antibody [RM0086-6A12] (ab86061)

    ab86061 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"