Overview

  • Product name
    Anti-Dhh antibody [RM0086-6A12]
    See all Dhh primary antibodies
  • Description
    Rat monoclonal [RM0086-6A12] to Dhh
  • Specificity
    ab86061 detects Mouse Dhh N terminal polypeptide, but not C terminal polypeptide.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse recombinant Dhh N-terminal fragment

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and filter sterilized through a 0.2 µm filter.
  • Clonality
    Monoclonal
  • Clone number
    RM0086-6A12
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Predicted molecular weight: Unprocessed precursor, 44 kDa; Dhh protein N-product, 20 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
    • Involvement in disease
      Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
      Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.
    • Sequence similarities
      Belongs to the hedgehog family.
    • Post-translational
      modifications
      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    • Cellular localization
      Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • C78960 antibody
      • Desert hedgehog (Drosophila) homolog antibody
      • Desert hedgehog antibody
      • Desert hedgehog homolog (Drosophila) antibody
      • Desert hedgehog protein C-product antibody
      • Desert hedgehog protein precursor antibody
      • DHH antibody
      • DHH_HUMAN antibody
      • GDXYM antibody
      • HHG-3 antibody
      • Hira antibody
      • SRXY7 antibody
      see all

    References

    This product has been referenced in:
    • Daane JM & Downs KM Hedgehog signaling in the posterior region of the mouse gastrula suggests manifold roles in the fetal-umbilical connection and posterior morphogenesis. Dev Dyn 240:2175-93 (2011). Read more (PubMed: 22016185) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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