Overview

  • Product nameAnti-DHODH antibody
    See all DHODH primary antibodies
  • Description
    Rabbit polyclonal to DHODH
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 179-228 (NLGKNKTSVD AAEDYAEGVR VLGPLADYLV VNVSSPNTAG LRSLQGKAEL) of Human DHODH (NP_001352)

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • FunctionCatalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
  • PathwayPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
  • Involvement in diseaseDefects in DHODH are the cause of postaxial acrofacial dysostosis (POADS) [MIM:263750]; also known as Miller syndrome. POADS is characterized by severe micrognathia, cleft lip and/or palate, hypoplasia or aplasia of the posterior elements of the limbs, coloboma of the eyelids and supernumerary nipples. POADS is a very rare disorder: only 2 multiplex families, each consisting of 2 affected siblings born to unaffected, nonconsanguineous parents, have been described among a total of around 30 reported cases.
  • Sequence similaritiesBelongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
  • Post-translational
    modifications
    The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHOdehase antibody
    • Dhodh antibody
    • Dihydroorotate dehydrogenase (quinone) antibody
    • Dihydroorotate dehydrogenase antibody
    • Dihydroorotate dehydrogenase mitochondrial antibody
    • Dihydroorotate oxidase antibody
    • Human complement of yeast URA1 antibody
    • mitochondrial antibody
    • POADS antibody
    • PYRD_HUMAN antibody
    • URA1 antibody
    see all

Anti-DHODH antibody images

  • Anti-DHODH antibody (ab83457) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DHODH antibody (ab83457)

ab83457 has not yet been referenced specifically in any publications.

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