Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
Protein modification; eIF5A hypusination.
Belongs to the deoxyhypusine synthase family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunoprecipitation of Hela whole cell lysates (0.5-1 mg per IP reaction; 20% of IP loaded) labeling DHPS with ab190266 at 1 µg/ml by western blot. DHPS was immunoprecipitated using ab190266 (lane 1) or control IgG (lane 2).